UBE4A was immunoprecipitated using: Lane A: 0.5 mg Jurkat Whole Cell Lysate. Lane B: 0.5 mg HepG2 Whole Cell Lysate. 4 uL anti-UBE4A rabbit polyclonal antibody and 15 ul of 50% Protein G agarose. Primary antibody: Anti-UBE4A rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 123 kDa. Observed band size: 130 kDa.
E4 / UBE4A Antibody (Preservative Free)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Immunoprecipitation, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$309.00each
Catalog Number: LS-C815733-100
Catalog Number: LS-C815733
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- Description
- UBE4A antibody LS-C815733 is an unconjugated rabbit polyclonal antibody to human UBE4A (E4). Validated for IP and WB.
- Application
- IP, WB
- Reactivity
- Hu
- Predicted Reactivity
- Rat
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- Ubiquitination factor E4A | UFD2 | UBOX2 | E4 | KIAA0126 | UBE4A
- Recommended Dilution: WB
- 1:500 - 1:2000
- UniProt Number
- Q14139
- NCBI GenBank
- NM_004788 | NP_004779.2
- SwissProt
- Q14139
Target
- Target
- Human E4 / UBE4A
- Antigen Species
- Human
- Epitope
- Human UBE4A
- Gene Name
- UBE4A
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- A synthetic peptide corresponding to the N-Terminus of human UBE4A
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.2 µm filtered solution in PBS
- Volume
- 100 Microliter
- Preservative
- No
- Purification Method
- Protein A Affinity
Storage & Handling
- Storage Conditions
- Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.
- Recommended Storage Buffer
- PBS