DIAPH3 / MDIA2 Antibody
Catalog Number: LS-C497059
2 sizes / from $281.00
Antibody: Rabbit Polyclonal
Applications: Western Blot
Reactivity: Human
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human DIAPH3 / MDIA2 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$153.00each
Catalog Number: LS-G92002-20
Catalog Number: LS-G92002
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human DIAPH3 (NM_030932) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- AUNA1 | DRF3 | Diap3 | Diaphanous-related formin-3 | DIA2 | DIAPH3 | MDia2 | NSDAN | Protein diaphanous homolog 3
- Origin Species
- Human
- NCBI GenBank
- NM_030932
- SwissProt
- Q9NSV4
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 98 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSEERSLSLL VKAVDPRHPN MMTDVVKLLS AVCIVGEESI LEEVLEALTS AGEEKKIDRF61FCIVEGLRHN SVQLQVACMQ LINALVTSPD DLDFRLHIRN EFMRCGLKEI LPNLKCIKND121GLDIQLKVFD EHKEEDLFEL SHRLEDIRAE LDEAYDVYNM VWSTVKETRA EGYFISILQH181LLLIRNDYFI RQQYFKLIDE CVSQIVLHRD GMDPDFTYRK RLDLDLTQFV DICIDQAKLE241EFEEKASELY KKFEKEFTDH QETQAELQKK EAKINELQAE LQAFKSQFGA LPADCNIPLP301PSKEGGTGHS ALPPPPPLPS GGGVPPPPPP PPPPPLPGMR MPFSGPVPPP PPLGFLGGQN361SPPLPILPFG LKPKKEFKPE ISMRRLNWLK IRPHEMTENC FWIKVNENKY ENVDLLCKLE421NTFCCQQKER REEEDIEEKK SIKKKIKELK FLDSKIAQNL SIFLSSFRVP YEEIRMMILE481VDETRLAESM IQNLIKHLPD QEQLNSLSQF KSEYSNLCEP EQFVVVMSNV KRLRPRLSAI541LFKLQFEEQV NNIKPDIMAV STACEEIKKS KSFSKLLELV LLMGNYMNAG SRNAQTFGFN601LSSLCKLKDT KSADQKTTLL HFLVEICEEK YPDILNFVDD LEPLDKASKV SVETLEKNLR661QMGRQLQQLE KELETFPPPE DLHDKFVTKM SRFVISAKEQ YETLSKLHEN MEKLYQSIIG721YYAIDVKKVS VEDFLTDLNN FRTTFMQAIK ENIKKREAEE KEKRVRIAKE LAERERLERQ781QKKKRLLEMK TEGDETGVMD NLLEALQSGA AFRDRRKRTP MPKDVRQSLS PMSQRPVLKV841CNHGNKPYLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
849 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
