Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
DIAPH3 / MDIA2 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G109341-100
Catalog Number: LS-G109341
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human DIAPH3 (NM_030932), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- AUNA1 | DRF3 | Diap3 | Diaphanous-related formin-3 | DIA2 | DIAPH3 | MDia2 | NSDAN | Protein diaphanous homolog 3
- Origin Species
- Human
- NCBI GenBank
- NM_030932
- SwissProt
- Q9NSV4
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 98 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSEERSLSLL VKAVDPRHPN MMTDVVKLLS AVCIVGEESI LEEVLEALTS AGEEKKIDRF61FCIVEGLRHN SVQLQVACMQ LINALVTSPD DLDFRLHIRN EFMRCGLKEI LPNLKCIKND121GLDIQLKVFD EHKEEDLFEL SHRLEDIRAE LDEAYDVYNM VWSTVKETRA EGYFISILQH181LLLIRNDYFI RQQYFKLIDE CVSQIVLHRD GMDPDFTYRK RLDLDLTQFV DICIDQAKLE241EFEEKASELY KKFEKEFTDH QETQAELQKK EAKINELQAE LQAFKSQFGA LPADCNIPLP301PSKEGGTGHS ALPPPPPLPS GGGVPPPPPP PPPPPLPGMR MPFSGPVPPP PPLGFLGGQN361SPPLPILPFG LKPKKEFKPE ISMRRLNWLK IRPHEMTENC FWIKVNENKY ENVDLLCKLE421NTFCCQQKER REEEDIEEKK SIKKKIKELK FLDSKIAQNL SIFLSSFRVP YEEIRMMILE481VDETRLAESM IQNLIKHLPD QEQLNSLSQF KSEYSNLCEP EQFVVVMSNV KRLRPRLSAI541LFKLQFEEQV NNIKPDIMAV STACEEIKKS KSFSKLLELV LLMGNYMNAG SRNAQTFGFN601LSSLCKLKDT KSADQKTTLL HFLVEICEEK YPDILNFVDD LEPLDKASKV SVETLEKNLR661QMGRQLQQLE KELETFPPPE DLHDKFVTKM SRFVISAKEQ YETLSKLHEN MEKLYQSIIG721YYAIDVKKVS VEDFLTDLNN FRTTFMQAIK ENIKKREAEE KEKRVRIAKE LAERERLERQ781QKKKRLLEMK TEGDETGVMD NLLEALQSGA AFRDRRKRTP MPKDVRQSLS PMSQRPVLKV841CNHGNKPYLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
849 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.