LSBio
Human CFAP52 / WDR16 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human CFAP52 / WDR16 Protein (Over-Expression Lysate Myc-DDK (Flag))

Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all CFAP52 antibodies →
$153.00each
Catalog Number: LS-G94116-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human WDR16(CFAP52) (NM_145054) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
WD repeat domain 16 | WDRPUH | WDR16
Origin Species
Human
SwissProt
Q8N1V2

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
68.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MDNKISPEAQ VAELELDAVI GFNGHVPTGL KCHPDQEHMI YPLGCTVLIQ AINTKEQNFL
61QGHGNNVSCL AISRSGEYIA SGQVTFMGFK ADIILWDYKN RELLARLSLH KGKIEALAFS
121PNDLYLVSLG GPDDGSVVVW SIAKRDAICG SPAAGLNVGN ATNVIFSRCR DEMFMTAGNG
181TIRVWELDLP NRKIWPTECQ TGQLKRIVMS IGVDDDDSFF YLGTTTGDIL KMNPRTKLLT
241DVGPAKDKFS LGVSAIRCLK MGGLLVGSGA GLLVFCKSPG YKPIKKIQLQ GGITSITLRG
301EGHQFLVGTE ESHIYRVSFT DFKETLIATC HFDAVEDIVF PFGTAELFAT CAKKDIRVWH
361TSSNRELLRI TVPNMTCHGI DFMRDGKSII SAWNDGKIRA FAPETGRLMY VINNAHRIGV
421TAIATTSDCK RVISGGGEGE VRVWQIGCQT QKLEEALKEH KSSVSCIRVK RNNEECVTAS
481TDGTCIIWDL VRLRRNQMIL ANTLFQCVCY HPEEFQIITS GTDRKIAYWE VFDGTVIREL
541EGSLSGSING MDITQEGVHF VTGGNDHLVK VWDYNEGEVT HVGVGHSGNI TRIRISPGNQ
601YIVSVSADGA ILRWKYPYTS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

620 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.