Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CFAP52 / WDR16 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G119704-100
Catalog Number: LS-G119704
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human WDR16(CFAP52) (NM_001080556), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- WD repeat domain 16 | WDRPUH | WDR16
- Origin Species
- Human
- SwissProt
- Q8N1V2
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 60.8 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MDNKISPEAQ VAELELDAVI GFNDIILWDY KNRELLARLS LHKGKIEALA FSPNDLYLVS61LGGPDDGSVV VWSIAKRDAI CGSPAAGLNV GNATNVIFSR CRDEMFMTAG NGTIRVWELD121LPNRKIWPTE CQTGQLKRIV MSIGVDDDDS FFYLGTTTGD ILKMNPRTKL LTDVGPAKDK181FSLGVSAIRC LKMGGLLVGS GAGLLVFCKS PGYKPIKKIQ LQGGITSITL RGEGHQFLVG241TEESHIYRVS FTDFKETLIA TCHFDAVEDI VFPFGTAELF ATCAKKDIRV WHTSSNRELL301RITVPNMTCH GIDFMRDGKS IISAWNDGKI RAFAPETGRL MYVINNAHRI GVTAIATTSD361CKRVISGGGE GEVRVWQIGC QTQKLEEALK EHKSSVSCIR VKRNNEECVT ASTDGTCIIW421DLVRLRRNQM ILANTLFQCV CYHPEEFQII TSGTDRKIAY WEVFDGTVIR ELEGSLSGSI481NGMDITQEGV HFVTGGNDHL VKVWDYNEGE VTHVGVGHSG NITRIRISPG NQYIVSVSAD541GAILRWKYPY TSTRTRPLEQKLISEEDLAANDILDYKDDDDKV
552 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.