Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CFAP52 / WDR16 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G106784-100
Catalog Number: LS-G106784
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human WDR16(CFAP52) (NM_145054), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- WD repeat domain 16 | WDRPUH | WDR16
- Origin Species
- Human
- SwissProt
- Q8N1V2
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 68.1 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MDNKISPEAQ VAELELDAVI GFNGHVPTGL KCHPDQEHMI YPLGCTVLIQ AINTKEQNFL61QGHGNNVSCL AISRSGEYIA SGQVTFMGFK ADIILWDYKN RELLARLSLH KGKIEALAFS121PNDLYLVSLG GPDDGSVVVW SIAKRDAICG SPAAGLNVGN ATNVIFSRCR DEMFMTAGNG181TIRVWELDLP NRKIWPTECQ TGQLKRIVMS IGVDDDDSFF YLGTTTGDIL KMNPRTKLLT241DVGPAKDKFS LGVSAIRCLK MGGLLVGSGA GLLVFCKSPG YKPIKKIQLQ GGITSITLRG301EGHQFLVGTE ESHIYRVSFT DFKETLIATC HFDAVEDIVF PFGTAELFAT CAKKDIRVWH361TSSNRELLRI TVPNMTCHGI DFMRDGKSII SAWNDGKIRA FAPETGRLMY VINNAHRIGV421TAIATTSDCK RVISGGGEGE VRVWQIGCQT QKLEEALKEH KSSVSCIRVK RNNEECVTAS481TDGTCIIWDL VRLRRNQMIL ANTLFQCVCY HPEEFQIITS GTDRKIAYWE VFDGTVIREL541EGSLSGSING MDITQEGVHF VTGGNDHLVK VWDYNEGEVT HVGVGHSGNI TRIRISPGNQ601YIVSVSADGA ILRWKYPYTSTRTRPLEQKLISEEDLAANDILDYKDDDDKV
620 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.