CDC27 Antibody
Catalog Number: LS-C403904
4 sizes / from $260.00
Antibody: Rabbit Polyclonal
Applications: Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general)
Reactivity: Human, Mouse, Rat
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human CDC27 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$220.00each
Catalog Number: LS-G78312-20
Catalog Number: LS-G78312
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human CDC27 (NM_001114091) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ANAPC3 | APC3 | CDC27 | CDC27 homolog | Cell division cycle 27 | D0S1430E | CDC27Hs | D17S978E | H-NUC | Nuc2 homolog | HNUC | NUC2
- Origin Species
- Human
- NCBI GenBank
- NM_001256
- SwissProt
- P30260
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 92.4 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MTVLQEPVQA AIWQALNHYA YRDAVFLAER LYAEVHSEEA LFLLATCYYR SGKAYKAYRL61LKGHSCTTPQ CKYLLAKCCV DLSKLAEGEQ ILSGGVFNKQ KSHDDIVTEF GDSACFTLSL121LGHVYCKTDR LAKGSECYQK SLSLNPFLWS PFESLCEIGE KPDPDQTFKF TSLQNFSNCL181PNSCTTQVPN HSLSHRQPET VLTETPQDTI ELNRLNLESS NSKYSLNTDS SVSYIDSAVI241SPDTVPLGTG TSILSKQVQN KPKTGRSLLG GPAALSPLTP SFGILPLETP SPGDGSYLQN301YTNTPPVIDV PSTGAPSKKT FRVLQSVARI GQTGTKSVFS QSGNSREVTP ILAQTQSSGP361QTSTTPQVLS PTITSPPNAL PRRSSRLFTS DSSTTKENSK KLKMKFPPKI PNRKTKSKTN421KGGITQPNIN DSLEITKLDS SIISEGKIST ITPQIQAFNL QKAAAEGLMS LLREMGKGYL481ALCSYNCKEA INILSHLPSH HYNTGWVLCQ IGRAYFELSE YMQAERIFSE VRRIENYRVE541GMEIYSTTLW HLQKDVALSV LSKDLTDMDK NSPEAWCAAG NCFSLQREHD IAIKFFQRAI601QVDPNYAYAY TLLGHEFVLT EELDKALACF RNAIRVNPRH YNAWYGLGMI YYKQEKFSLA661EMHFQKALDI NPQSSVLLCH IGVVQHALKK SEKALDTLNK AIVIDPKNPL CKFHRASVLF721ANEKYKSALQ ELEELKQIVP KESLVYFLIG KVYKKLGQTH LALMNFSWAM DLDPKGANNQ781IKEAIDKRYL PDDEEPITQE EQIMGTDESQ ESSMTDADDT QLHAAESDEFTRTRPLEQKLISEEDLAANDILDYKDDDDKV
830 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
