CDC27 Antibody (phospho-Ser426)

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Antibody:	Rabbit Polyclonal
Applications:	Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunoprecipitation, Western Blot
Reactivity:	Human
Format:	Liquid

$490.00each

Catalog Number: LS-C18876-100

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Description: CDC27 antibody LS-C18876 is an unconjugated rabbit polyclonal antibody to CDC27 (pSer426) from human. It is reactive with human, rat, chicken and other species. Validated for ELISA, IP and WB.
Application: ELISA, IHC, IP, WB
Reactivity: Hu
Predicted Reactivity: Chicken, Dog, Non-Human Primate, Rat
Intended Use: Research Use Only
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names: ANAPC3 | APC3 | CDC27 | CDC27 homolog | Cell division cycle 27 | D0S1430E | CDC27Hs | D17S978E | H-NUC | Nuc2 homolog | HNUC | NUC2
Recommended Dilution: ELISA: 1:5000 - 1:25000
Recommended Dilution: IP: 1:100
Recommended Dilution: WB: 1:500 - 1:2500
UniProt Number: P30260
NCBI GenBank: NM_001256 | NP_001247.3
SwissProt: P30260

Target: Human CDC27
Antigen Species: Human
Epitope: Reactivity occurs against human CDC27 pS426 protein and the antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated human CDC27 is minimal by ELISA. The antibody does not cross-react with CDC27 phosphorylated at other sites. A BLAST analysis was used to suggest reactivity with this protein from human, rat, dog, chicken and chimpanzee based on 100% homology for the immunogen sequence. Cross reactivity with CDC27 protein from mouse may occur as sequence homology varies by one amino acid residue in this sequence (89% homology). Cross reactivity with CDC27 homologues from other sources has not been determined.
Gene Name: CDC27

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal portion of Human cdc27 amino acids 415-445 surrounding S426.
Immunogen Type: Synthetic Peptide (Linear)

Conjugate/Label: Unconjugated
Format: Liquid
Formulation: 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration: 0.99 mg/mL
Preservative: Yes
Purification Method: Affinity Purified

Storage Conditions: Store vial at -20°C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
Recommended Storage Buffer: 0.02 M Potassium Phosphate|0.15 M NaCl