LSBio
CDC27 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CDC27 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all CDC27 antibodies →
$725.00each
Catalog Number: LS-G124319-100

For bulk orders or custom formulations:

Request a Quote

Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CDC27 (NM_001114091), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ANAPC3 | APC3 | CDC27 | CDC27 homolog | Cell division cycle 27 | D0S1430E | CDC27Hs | D17S978E | H-NUC | Nuc2 homolog | HNUC | NUC2
Origin Species
Human
NCBI GenBank
NM_001256
SwissProt
P30260

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
92.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MTVLQEPVQA AIWQALNHYA YRDAVFLAER LYAEVHSEEA LFLLATCYYR SGKAYKAYRL
61LKGHSCTTPQ CKYLLAKCCV DLSKLAEGEQ ILSGGVFNKQ KSHDDIVTEF GDSACFTLSL
121LGHVYCKTDR LAKGSECYQK SLSLNPFLWS PFESLCEIGE KPDPDQTFKF TSLQNFSNCL
181PNSCTTQVPN HSLSHRQPET VLTETPQDTI ELNRLNLESS NSKYSLNTDS SVSYIDSAVI
241SPDTVPLGTG TSILSKQVQN KPKTGRSLLG GPAALSPLTP SFGILPLETP SPGDGSYLQN
301YTNTPPVIDV PSTGAPSKKT FRVLQSVARI GQTGTKSVFS QSGNSREVTP ILAQTQSSGP
361QTSTTPQVLS PTITSPPNAL PRRSSRLFTS DSSTTKENSK KLKMKFPPKI PNRKTKSKTN
421KGGITQPNIN DSLEITKLDS SIISEGKIST ITPQIQAFNL QKAAAEGLMS LLREMGKGYL
481ALCSYNCKEA INILSHLPSH HYNTGWVLCQ IGRAYFELSE YMQAERIFSE VRRIENYRVE
541GMEIYSTTLW HLQKDVALSV LSKDLTDMDK NSPEAWCAAG NCFSLQREHD IAIKFFQRAI
601QVDPNYAYAY TLLGHEFVLT EELDKALACF RNAIRVNPRH YNAWYGLGMI YYKQEKFSLA
661EMHFQKALDI NPQSSVLLCH IGVVQHALKK SEKALDTLNK AIVIDPKNPL CKFHRASVLF
721ANEKYKSALQ ELEELKQIVP KESLVYFLIG KVYKKLGQTH LALMNFSWAM DLDPKGANNQ
781IKEAIDKRYL PDDEEPITQE EQIMGTDESQ ESSMTDADDT QLHAAESDEF
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

830 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.