LSBio
Human BRN2 / POU3F2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Human BRN2 / POU3F2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all POU3F2 antibodies →
$153.00each
Catalog Number: LS-G77629-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human POU3F2 (NM_005604) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Brain-2 | Brn-2 | BRN2 | Oct-7 | Octamer-binding protein 7 | OTF7 | POU class 3 homeobox 2 | N-Oct3 | POU3F2 | OCT7 | OTF-7 | POUF3
Origin Species
Human
NCBI GenBank
NM_005604
SwissProt
P20265

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
46.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MATAASNHYS LLTSSASIVH AEPPGGMQQG AGGYREAQSL VQGDYGALQS NGHPLSHAHQ
61WITALSHGGG GGGGGGGGGG GGGGGGGGDG SPWSTSPLGQ PDIKPSVVVQ QGGRGDELHG
121PGALQQQHQQ QQQQQQQQQQ QQQQQQQQQR PPHLVHHAAN HHPGPGAWRS AAAAAHLPPS
181MGASNGGLLY SQPSFTVNGM LGAGGQPAGL HHHGLRDAHD EPHHADHHPH PHSHPHQQPP
241PPPPPQGPPG HPGAHHDPHS DEDTPTSDDL EQFAKQFKQR RIKLGFTQAD VGLALGTLYG
301NVFSQTTICR FEALQLSFKN MCKLKPLLNK WLEEADSSSG SPTSIDKIAA QGRKRKKRTS
361IEVSVKGALE SHFLKCPKPS AQEITSLADS LQLEKEVVRV WFCNRRQKEK RMTPPGGTLP
421GAEDVYGGSR DTPPHHGVQT PVQ
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

443 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.