Detection of Human Brn-2 by Western Blot. Samples: Whole cell lysate from 293T (15 and 50 ug), Jurkat (J; 50 ug) and HeLa (H; 50 ug) cells. Antibodies: Affinity purified rabbit anti-Brn-2 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
BRN2 / POU3F2 Antibody (aa393-443)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$301.00each
Catalog Number: LS-C286175-10
Catalog Number: LS-C286175
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- Description
- POU3F2 antibody LS-C286175 is an unconjugated rabbit polyclonal antibody to human POU3F2 (BRN2) (aa393-443). Validated for WB.
- Application
- WB
- Reactivity
- Hu
- Predicted Reactivity
- Mouse, Rat, Zebrafish
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- Brain-2 | Brn-2 | BRN2 | Oct-7 | Octamer-binding protein 7 | OTF7 | POU class 3 homeobox 2 | N-Oct3 | POU3F2 | OCT7 | OTF-7 | POUF3
- Recommended Dilution: WB
- 1:2000 - 1:10000
- UniProt Number
- P20265
- NCBI GenBank
- NM_005604 | NP_005595.2
- SwissProt
- P20265
Target
- Target
- Human BRN2 / POU3F2
- Antigen Species
- Human
- Epitope
- Region between residue 393 and 443 of human Brain-specific Homeobox/POU Domain Protein 2 using the numbering given in entry NP_005595.2 (GeneID 5454).
- Gene Name
- POU3F2
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- A portion of GeneID: 5454 corresponding to amino acids between 393 and 443 of UniprotID: P20265
- Immunogen Type
- Recombinant Protein Fragment
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- Tris-citrate/phosphate buffer, pH 7-8, 0.09% sodium azide.
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at 2-8°C for up to one year.
- Recommended Storage Buffer
- Tris-citrate