BRN2 / POU3F2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G115859-100
Catalog Number: LS-G115859
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human POU3F2 (NM_005604), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Brain-2 | Brn-2 | BRN2 | Oct-7 | Octamer-binding protein 7 | OTF7 | POU class 3 homeobox 2 | N-Oct3 | POU3F2 | OCT7 | OTF-7 | POUF3
- Origin Species
- Human
- NCBI GenBank
- NM_005604
- SwissProt
- P20265
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 46.7 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MATAASNHYS LLTSSASIVH AEPPGGMQQG AGGYREAQSL VQGDYGALQS NGHPLSHAHQ61WITALSHGGG GGGGGGGGGG GGGGGGGGDG SPWSTSPLGQ PDIKPSVVVQ QGGRGDELHG121PGALQQQHQQ QQQQQQQQQQ QQQQQQQQQR PPHLVHHAAN HHPGPGAWRS AAAAAHLPPS181MGASNGGLLY SQPSFTVNGM LGAGGQPAGL HHHGLRDAHD EPHHADHHPH PHSHPHQQPP241PPPPPQGPPG HPGAHHDPHS DEDTPTSDDL EQFAKQFKQR RIKLGFTQAD VGLALGTLYG301NVFSQTTICR FEALQLSFKN MCKLKPLLNK WLEEADSSSG SPTSIDKIAA QGRKRKKRTS361IEVSVKGALE SHFLKCPKPS AQEITSLADS LQLEKEVVRV WFCNRRQKEK RMTPPGGTLP421GAEDVYGGSR DTPPHHGVQT PVQTRTRPLEQKLISEEDLAANDILDYKDDDDKV
443 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.