BLNK Antibody
Catalog Number: LS-C830419
4 sizes / from $260.00
Antibody: Rabbit Polyclonal
Applications: Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot
Reactivity: Human, Mouse, Rat
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human BLNK Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$153.00each
Catalog Number: LS-G89799-20
Catalog Number: LS-G89799
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human BLNK (NM_001114094) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- AGM4 | BLNK-S | B-cell activation | B-cell linker protein | BLNK | B cell linker protein | Bca | Cytoplasmic adapter protein | LY57 | SLP-65 | SLP65 | B-cell linker | BASH
- Origin Species
- Human
- NCBI GenBank
- NM_013314
- SwissProt
- Q8WV28
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 48 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MDKLNKITVP ASQKLRQLQK MVHDIKNNEG GIMNKIKKLK VKAPPSVPRR DYASESPADE61EEQWSDDFDS DYENPDEHSD SEMYVMPAEE NADDSYEPPP VEQETRPVHP ALPFARGEYI121DNRSSQRHSP PFSKTLPSKP SWPSEKARLT STLPALTALQ KPQVPPKPKG LLEDEADYVV181PVEDNDENYI HPTESSSPPP EKGRNSGAWE TKSPPPAAPS PLPRAGKKPT TPLKTTPVAS241QQNASSVCEE KPIPAERHRG SSHRQEAVQS PVFPPAQKQI HQKPIPLPRF TEGGNPTVDG301PLPSFSSNST ISEQEAGVLC KPWYAGACDR KSAEEALHRS NKDGSFLIRK SSGHDSKQPY361TLVVFFNKRV YNIPVRFIEA TKQYALGRKK NGEEYFGSVA EIIRNHQHSP LVLIDSQNNT421KDSTRLKYAV KVSTRTRPLEQKLISEEDLAANDILDYKDDDDKV
433 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
