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BLNK Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

BLNK Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G124323-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human BLNK (NM_001114094), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
AGM4 | BLNK-S | B-cell activation | B-cell linker protein | BLNK | B cell linker protein | Bca | Cytoplasmic adapter protein | LY57 | SLP-65 | SLP65 | B-cell linker | BASH
Origin Species
Human
NCBI GenBank
NM_013314
SwissProt
Q8WV28

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
48 kDa
Protein Type
Lysate
Amino Acid Sequence
1MDKLNKITVP ASQKLRQLQK MVHDIKNNEG GIMNKIKKLK VKAPPSVPRR DYASESPADE
61EEQWSDDFDS DYENPDEHSD SEMYVMPAEE NADDSYEPPP VEQETRPVHP ALPFARGEYI
121DNRSSQRHSP PFSKTLPSKP SWPSEKARLT STLPALTALQ KPQVPPKPKG LLEDEADYVV
181PVEDNDENYI HPTESSSPPP EKGRNSGAWE TKSPPPAAPS PLPRAGKKPT TPLKTTPVAS
241QQNASSVCEE KPIPAERHRG SSHRQEAVQS PVFPPAQKQI HQKPIPLPRF TEGGNPTVDG
301PLPSFSSNST ISEQEAGVLC KPWYAGACDR KSAEEALHRS NKDGSFLIRK SSGHDSKQPY
361TLVVFFNKRV YNIPVRFIEA TKQYALGRKK NGEEYFGSVA EIIRNHQHSP LVLIDSQNNT
421KDSTRLKYAV KVS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

433 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.