LSBio
Human BLIMP1 / PRDM1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human BLIMP1 / PRDM1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all PRDM1 antibodies →
$220.00each
Catalog Number: LS-G89109-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human PRDM1(PRDM1) (NM_001198) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
BLIMP-1 | BLIMP1 | PRDI-binding factor-1 | PRDI-BF1 | PR domain-containing protein 1 | PRDI-binding factor 1 | PRDM1
Origin Species
Human
NCBI GenBank
NM_001198
SwissProt
O75626

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
88.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MKMDMEDADM TLWTEAEFEE KCTYIVNDHP WDSGADGGTS VQAEASLPRN LLFKYATNSE
61EVIGVMSKEY IPKGTRFGPL IGEIYTNDTV PKNANRKYFW RIYSRGELHH FIDGFNEEKS
121NWMRYVNPAH SPREQNLAAC QNGMNIYFYT IKPIPANQEL LVWYCRDFAE RLHYPYPGEL
181TMMNLTQTQS SLKQPSTEKN ELCPKNVPKR EYSVKEILKL DSNPSKGKDL YRSNISPLTS
241EKDLDDFRRR GSPEMPFYPR VVYPIRAPLP EDFLKASLAY GIERPTYITR SPIPSSTTPS
301PSARSSPDQS LKSSSPHSSP GNTVSPVGPG SQEHRDSYAY LNASYGTEGL GSYPGYAPLP
361HLPPAFIPSY NAHYPKFLLP PYGMNCNGLS AVSSMNGINN FGLFPRLCPV YSNLLGGGSL
421PHPMLNPTSL PSSLPSDGAR RLLQPEHPRE VLVPAPHSAF SFTGAAASMK DKACSPTSGS
481PTAGTAATAE HVVQPKATSA AMAAPSSDEA MNLIKNKRNM TGYKTLPYPL KKQNGKIKYE
541CNVCAKTFGQ LSNLKVHLRV HSGERPFKCQ TCNKGFTQLA HLQKHYLVHT GEKPHECQVC
601HKRFSSTSNL KTHLRLHSGE KPYQCKVCPA KFTQFVHLKL HKRLHTRERP HKCSQCHKNY
661IHLCSLKVHL KGNCAAAPAP GLPLEDLTRI NEEIEKFDIS DNADRLEDVE DDISVISVVE
721KEILAVVRKE KEETGLKVSL QRNMGNGLLS SGCSLYESSD LPLMKLPPSN PLPLVPVKVK
781QETVEPMDPS GP
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

792 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.