Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
BLIMP1 / PRDM1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G99362-100
Catalog Number: LS-G99362
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PRDM1(PRDM1) (NM_001198), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- BLIMP-1 | BLIMP1 | PRDI-binding factor-1 | PRDI-BF1 | PR domain-containing protein 1 | PRDI-binding factor 1 | PRDM1
- NCBI GenBank
- NM_001198
- SwissProt
- O75626
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 88.4 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MKMDMEDADM TLWTEAEFEE KCTYIVNDHP WDSGADGGTS VQAEASLPRN LLFKYATNSE61EVIGVMSKEY IPKGTRFGPL IGEIYTNDTV PKNANRKYFW RIYSRGELHH FIDGFNEEKS121NWMRYVNPAH SPREQNLAAC QNGMNIYFYT IKPIPANQEL LVWYCRDFAE RLHYPYPGEL181TMMNLTQTQS SLKQPSTEKN ELCPKNVPKR EYSVKEILKL DSNPSKGKDL YRSNISPLTS241EKDLDDFRRR GSPEMPFYPR VVYPIRAPLP EDFLKASLAY GIERPTYITR SPIPSSTTPS301PSARSSPDQS LKSSSPHSSP GNTVSPVGPG SQEHRDSYAY LNASYGTEGL GSYPGYAPLP361HLPPAFIPSY NAHYPKFLLP PYGMNCNGLS AVSSMNGINN FGLFPRLCPV YSNLLGGGSL421PHPMLNPTSL PSSLPSDGAR RLLQPEHPRE VLVPAPHSAF SFTGAAASMK DKACSPTSGS481PTAGTAATAE HVVQPKATSA AMAAPSSDEA MNLIKNKRNM TGYKTLPYPL KKQNGKIKYE541CNVCAKTFGQ LSNLKVHLRV HSGERPFKCQ TCNKGFTQLA HLQKHYLVHT GEKPHECQVC601HKRFSSTSNL KTHLRLHSGE KPYQCKVCPA KFTQFVHLKL HKRLHTRERP HKCSQCHKNY661IHLCSLKVHL KGNCAAAPAP GLPLEDLTRI NEEIEKFDIS DNADRLEDVE DDISVISVVE721KEILAVVRKE KEETGLKVSL QRNMGNGLLS SGCSLYESSD LPLMKLPPSN PLPLVPVKVK781QETVEPMDPS GPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
792 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.