Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
BLIMP1 / PRDM1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G104085-100
Catalog Number: LS-G104085
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PRDM1(PRDM1) (NM_182907), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- BLIMP-1 | BLIMP1 | PRDI-binding factor-1 | PRDI-BF1 | PR domain-containing protein 1 | PRDI-binding factor 1 | PRDM1
- Origin Species
- Human
- NCBI GenBank
- NM_001198
- SwissProt
- O75626
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 76.7 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MEKIYSRGEL HHFIDGFNEE KSNWMRYVNP AHSPREQNLA ACQNGMNIYF YTIKPIPANQ61ELLVWYCRDF AERLHYPYPG ELTMMNLTQT QSSLKQPSTE KNELCPKNVP KREYSVKEIL121KLDSNPSKGK DLYRSNISPL TSEKDLDDFR RRGSPEMPFY PRVVYPIRAP LPEDFLKASL181AYGIERPTYI TRSPIPSSTT PSPSARSSPD QSLKSSSPHS SPGNTVSPVG PGSQEHRDSY241AYLNASYGTE GLGSYPGYAP LPHLPPAFIP SYNAHYPKFL LPPYGMNCNG LSAVSSMNGI301NNFGLFPRLC PVYSNLLGGG SLPHPMLNPT SLPSSLPSDG ARRLLQPEHP REVLVPAPHS361AFSFTGAAAS MKDKACSPTS GSPTAGTAAT AEHVVQPKAT SAAMAAPSSD EAMNLIKNKR421NMTGYKTLPY PLKKQNGKIK YECNVCAKTF GQLSNLKVHL RVHSGERPFK CQTCNKGFTQ481LAHLQKHYLV HTGEKPHECQ VCHKRFSSTS NLKTHLRLHS GEKPYQCKVC PAKFTQFVHL541KLHKRLHTRE RPHKCSQCHK NYIHLCSLKV HLKGNCAAAP APGLPLEDLT RINEEIEKFD601ISDNADRLED VEDDISVISV VEKEILAVVR KEKEETGLKV SLQRNMGNGL LSSGCSLYES661SDLPLMKLPP SNPLPLVPVK VKQETVEPMD PSGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
691 aa + 34 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.