ARHGEF7 Antibody
Catalog Number: LS-C331262
2 sizes / from $281.00
Antibody: Rabbit Polyclonal
Applications: Immunohistochemistry (general), Western Blot
Reactivity: Human, Mouse, Rat
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human ARHGEF7 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$220.00each
Catalog Number: LS-G76674-20
Catalog Number: LS-G76674
For bulk orders or custom formulations:
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human ARHGEF7 (NM_145735) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ARHGEF7 | COOL1 | COOL-1 | Hypothetical protein kiaa0142 | Nbla10314 | p85 | p85COOL1 | p85SPR | PIXB | KIAA0142 | p50 | PAK3BP | BETA-PIX | p50BP
- Origin Species
- Human
- NCBI GenBank
- NM_003899
- SwissProt
- Q14155
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 87.6 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MNSAEQTVTW LITLGVLESP KKTISDPEGF LQASLKDGVV LCRLLERLLP GTIEKVYPEP61RSESECLSNI REFLRGCGAS LRLETFDAND LYQGQNFNKV LSSLVTLNKV TADIGLGSDS121VCARPSSHRI KSFDSLGSQS LHTRTSKLFQ GQYRSLDMTD NSNNQLVVRA KFNFQQTNED181ELSFSKGDVI HVTRVEEGGW WEGTLNGRTG WFPSNYVREV KASEKPVSPK SGTLKSPPKG241FDTTAINKSY YNVVLQNILE TENEYSKELQ TVLSTYLRPL QTSEKLSSAN ISYLMGNLEE301ICSFQQMLVQ SLEECTKLPE AQQRVGGCFL NLMPQMKTLY LTYCANHPSA VNVLTEHSEE361LGEFMETKGA SSPGILVLTT GLSKPFMRLD KYPTLLKELE RHMEDYHTDR QDIQKSMAAF421KNLSAQCQEV RKRKELELQI LTEAIRNWEG DDIKTLGNVT YMSQVLIQCA GSEEKNERYL481LLFPNVLLML SASPRMSGFI YQGKLPTTGM TITKLEDSEN HRNAFEISGS MIERILVSCN541NQQDLQEWVE HLQKQTKVTS VGNPTIKPHS VPSHTLPSHP VTPSSKHADS KPAPLTPAYH601TLPHPSHHGT PHTTINWGPL EPPKTPKPWS LSCLRPAPPL RPSAALCYKE DLSKSPKTMK661KLLPKRKPER KPSDEEFASR KSTAALEEDA QILKVIEAYC TSAKTRQTLN STWQGTDLMH721NHVLADDDQP SLDSLGRRSS LSRLEPSDLS EDSDYDSIWT AHSYRMGSTS RKSCCSYISH781QNTRTRPLEQKLISEEDLAANDILDYKDDDDKV
782 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
