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ARHGEF7 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ARHGEF7 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ARHGEF7 antibodies →
$725.00each
Catalog Number: LS-G117023-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ARHGEF7 (NM_003899), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ARHGEF7 | COOL1 | COOL-1 | Hypothetical protein kiaa0142 | Nbla10314 | p85 | p85COOL1 | p85SPR | PIXB | KIAA0142 | p50 | PAK3BP | BETA-PIX | p50BP
Origin Species
Human
NCBI GenBank
NM_003899
SwissProt
Q14155

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
73 kDa
Protein Type
Lysate
Amino Acid Sequence
1MTDNSNNQLV VRAKFNFQQT NEDELSFSKG DVIHVTRVEE GGWWEGTLNG RTGWFPSNYV
61REVKASEKPV SPKSGTLKSP PKGFDTTAIN KSYYNVVLQN ILETENEYSK ELQTVLSTYL
121RPLQTSEKLS SANISYLMGN LEEICSFQQM LVQSLEECTK LPEAQQRVGG CFLNLMPQMK
181TLYLTYCANH PSAVNVLTEH SEELGEFMET KGASSPGILV LTTGLSKPFM RLDKYPTLLK
241ELERHMEDYH TDRQDIQKSM AAFKNLSAQC QEVRKRKELE LQILTEAIRN WEGDDIKTLG
301NVTYMSQVLI QCAGSEEKNE RYLLLFPNVL LMLSASPRMS GFIYQGKLPT TGMTITKLED
361SENHRNAFEI SGSMIERILV SCNNQQDLQE WVEHLQKQTK VTSVGNPTIK PHSVPSHTLP
421SHPVTPSSKH ADSKPAPLTP AYHTLPHPSH HGTPHTTINW GPLEPPKTPK PWSLSCLRPA
481PPLRPSAALC YKEDLSKSPK TMKKLLPKRK PERKPSDEEF ASRKSTAALE EDAQILKVIE
541AYCTSAKTRQ TLNSSSRKES APQVLLPEEE KIIVEETKSN GQTVIEEKSL VDTVYALKDE
601VQELRQDNKK MKKSLEEEQR ARKDLEKLVR KVLKNMNDPA WDETNL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

646 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.