LSBio
ARHGEF7 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ARHGEF7 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ARHGEF7 antibodies →
$725.00each
Catalog Number: LS-G106587-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ARHGEF7 (NM_145735), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ARHGEF7 | COOL1 | COOL-1 | Hypothetical protein kiaa0142 | Nbla10314 | p85 | p85COOL1 | p85SPR | PIXB | KIAA0142 | p50 | PAK3BP | BETA-PIX | p50BP
Origin Species
Human
NCBI GenBank
NM_003899
SwissProt
Q14155

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
87.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MNSAEQTVTW LITLGVLESP KKTISDPEGF LQASLKDGVV LCRLLERLLP GTIEKVYPEP
61RSESECLSNI REFLRGCGAS LRLETFDAND LYQGQNFNKV LSSLVTLNKV TADIGLGSDS
121VCARPSSHRI KSFDSLGSQS LHTRTSKLFQ GQYRSLDMTD NSNNQLVVRA KFNFQQTNED
181ELSFSKGDVI HVTRVEEGGW WEGTLNGRTG WFPSNYVREV KASEKPVSPK SGTLKSPPKG
241FDTTAINKSY YNVVLQNILE TENEYSKELQ TVLSTYLRPL QTSEKLSSAN ISYLMGNLEE
301ICSFQQMLVQ SLEECTKLPE AQQRVGGCFL NLMPQMKTLY LTYCANHPSA VNVLTEHSEE
361LGEFMETKGA SSPGILVLTT GLSKPFMRLD KYPTLLKELE RHMEDYHTDR QDIQKSMAAF
421KNLSAQCQEV RKRKELELQI LTEAIRNWEG DDIKTLGNVT YMSQVLIQCA GSEEKNERYL
481LLFPNVLLML SASPRMSGFI YQGKLPTTGM TITKLEDSEN HRNAFEISGS MIERILVSCN
541NQQDLQEWVE HLQKQTKVTS VGNPTIKPHS VPSHTLPSHP VTPSSKHADS KPAPLTPAYH
601TLPHPSHHGT PHTTINWGPL EPPKTPKPWS LSCLRPAPPL RPSAALCYKE DLSKSPKTMK
661KLLPKRKPER KPSDEEFASR KSTAALEEDA QILKVIEAYC TSAKTRQTLN STWQGTDLMH
721NHVLADDDQP SLDSLGRRSS LSRLEPSDLS EDSDYDSIWT AHSYRMGSTS RKSCCSYISH
781QN
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

782 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.