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Human ADGRE5 / CD97 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human ADGRE5 / CD97 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all ADGRE5 antibodies →
$153.00each
Catalog Number: LS-G75104-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human CD97(ADGRE5) (NM_001784) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CD97 | CD97 molecule | Leukocyte antigen CD97 | TM7LN1 | CD97 antigen
Origin Species
Human
NCBI GenBank
NM_078481
SwissProt
P48960

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
81.74 kDa
Protein Type
Lysate
Amino Acid Sequence
1MGGRVFLAFC VWLTLPGAET QDSRGCARWC PQNSSCVNAT ACRCNPGFSS FSEIITTPTE
61TCDDINECAT PSKVSCGKFS DCWNTEGSYD CVCSPGYEPV SGAKTFKNES ENTCQDVDEC
121SSGQHQCDSS TVCFNTVGSY SCRCRPGWKP RHGIPNNQKD TVCEDMTFST WTPPPGVHSQ
181TLSRFFDKVQ DLGRDSKTSS AEVTIQNVIK LVDELMEAPG DVEALAPPVR HLIATQLLSN
241LEDIMRILAK SLPKGPFTYI SPSNTELTLM IQERGDKNVT MGQSSARMKL NWAVAAGAED
301PGPAVAGILS IQNMTTLLAN ASLNLHSKKQ AELEEIYESS IRGVQLRRLS AVNSIFLSHN
361NTKELNSPIL FAFSHLESSD GEAGRDPPAK DVMPGPRQEL LCAFWKSDSD RGGHWATEGC
421QVLGSKNGST TCQCSHLSSF AILMAHYDVE DWKLTLITRV GLALSLFCLL LCILTFLLVR
481PIQGSRTTIH LHLCICLFVG STIFLAGIEN EGGQVGLRCR LVAGLLHYCF LAAFCWMSLE
541GLELYFLVVR VFQGQGLSTR WLCLIGYGVP LLIVGVSAAI YSKGYGRPRY CWLDFEQGFL
601WSFLGPVTFI ILCNAVIFVT TVWKLTQKFS EINPDMKKLK KARALTITAI AQLFLLGCTW
661VFGLFIFDDR SLVLTYVFTI LNCLQGAFLY LLHCLLNKKV REEYRKWACL VAGGSKYSEF
721TSTTSGTGHN QTRALRASES GI
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

742 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.