Polyclonal Rabbit anti-Human SLC5A11 / SMIT2 Antibody (WB)
Catalog Number: LS-C748223
2 sizes / from $281.00
Antibody: Rabbit Polyclonal
Applications: Immunohistochemistry (general), Western Blot
Reactivity: Human, Mouse, Rat
Format: Liquid
Anti-CD97 Antibody - Western Blot. Western blot of Protein A purified anti-CD97 antibody shows detection of bands corresponding to free Fc-CD97- (5EGF) (lower arrowhead) and Fc-CD97- (5EGF) present as a complex (upper arrowhead) in lysates from COS cells. The left lane contains lysate from cells transfected with control DNA. The right lane contains lysate from COS cells expressing Fc-CD97- (5EGF). No staining was noted from bone marrow lysates taken from CD97 knockout mice. The identity of the band at ~65 kD appearing in all lanes is not known. The formation of the CD97 complex is currently under investigation. Approximately 10 ul of lysate was used in each lane. A 1:1000 dilution of the primary antibody was used. The image was processed using a 10-sec exposure. Personal Communication. Yvona Ward. NIH, NCI, CCR, Bethesda, MD.
Polyclonal Rabbit anti-Mouse ADGRE5 / CD97 Antibody (IF, WB)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Immunohistochemistry (general), Western Blot |
| Reactivity: | Human, Mouse, Non-Human Primate |
| Format: | Lyophilized |
$619.00each
Catalog Number: LS-C18770-500
Catalog Number: LS-C18770
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- CD97 antibody LS-C18770 is an unconjugated rabbit polyclonal antibody to CD97 (ADGRE5) from mouse. It is reactive with human, mouse and chimpanzee. Validated for ELISA, IF and WB.
- Application
- ELISA, IF, IHC, WB
- Reactivity
- Hu, Ms, NHP
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- CD97 | CD97 molecule | Leukocyte antigen CD97 | TM7LN1 | CD97 antigen
- Recommended Dilution: ELISA
- 1:10000 - 1:50000
- Recommended Dilution: IF/ICC
- 1:1000 - 1:5000
- Recommended Dilution: WB
- 1:1000 - 1:5000
- UniProt Number
- P48960
- NCBI GenBank
- NM_078481 | NP_510966.1
- SwissProt
- P48960
Target
- Target
- Mouse ADGRE5 / CD97
- Antigen Species
- Mouse
- Gene Name
- ADGRE5
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to amino acids 1-512 (extracellular domain) of mouse CD97 protein.
- Immunogen Type
- Recombinant Protein Fragment
Format
- Conjugate/Label
- Unconjugated
- Format
- Lyophilized
- Formulation
- Lyophilized from 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 5 mg/mL
- Preservative
- Yes
- Purification Method
- Protein A Affinity
Storage & Handling
- Storage Conditions
- Store vial at 4°C prior to opening. Restore with 0.1 ml of deionized water or equivalent. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl
