Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ADGRE5 / CD97 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G99557-100
Catalog Number: LS-G99557
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CD97(ADGRE5) (NM_001784), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CD97 | CD97 molecule | Leukocyte antigen CD97 | TM7LN1 | CD97 antigen
- NCBI GenBank
- NM_078481
- SwissProt
- P48960
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 81.74 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MGGRVFLAFC VWLTLPGAET QDSRGCARWC PQNSSCVNAT ACRCNPGFSS FSEIITTPTE61TCDDINECAT PSKVSCGKFS DCWNTEGSYD CVCSPGYEPV SGAKTFKNES ENTCQDVDEC121SSGQHQCDSS TVCFNTVGSY SCRCRPGWKP RHGIPNNQKD TVCEDMTFST WTPPPGVHSQ181TLSRFFDKVQ DLGRDSKTSS AEVTIQNVIK LVDELMEAPG DVEALAPPVR HLIATQLLSN241LEDIMRILAK SLPKGPFTYI SPSNTELTLM IQERGDKNVT MGQSSARMKL NWAVAAGAED301PGPAVAGILS IQNMTTLLAN ASLNLHSKKQ AELEEIYESS IRGVQLRRLS AVNSIFLSHN361NTKELNSPIL FAFSHLESSD GEAGRDPPAK DVMPGPRQEL LCAFWKSDSD RGGHWATEGC421QVLGSKNGST TCQCSHLSSF AILMAHYDVE DWKLTLITRV GLALSLFCLL LCILTFLLVR481PIQGSRTTIH LHLCICLFVG STIFLAGIEN EGGQVGLRCR LVAGLLHYCF LAAFCWMSLE541GLELYFLVVR VFQGQGLSTR WLCLIGYGVP LLIVGVSAAI YSKGYGRPRY CWLDFEQGFL601WSFLGPVTFI ILCNAVIFVT TVWKLTQKFS EINPDMKKLK KARALTITAI AQLFLLGCTW661VFGLFIFDDR SLVLTYVFTI LNCLQGAFLY LLHCLLNKKV REEYRKWACL VAGGSKYSEF721TSTTSGTGHN QTRALRASES GITRTRPLEQKLISEEDLAANDILDYKDDDDKV
742 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.