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Human ADGRE2 / EMR2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human ADGRE2 / EMR2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all ADGRE2 antibodies →
$220.00each
Catalog Number: LS-G88958-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human EMR2(EMR2) (NM_152918) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CD312 antigen | EMR2 | EGF-like module receptor 2 | CD312
Origin Species
Human
NCBI GenBank
NM_013447
SwissProt
Q9UHX3

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
74.8 kda
Protein Type
Lysate
Amino Acid Sequence
1MGGRVFLVFL AFCVWLTLPG AETQDSRGCA RWCPQDSSCV NATACRCNPG FSSFSEIITT
61PMETCDDINE CATLSKVSCG KFSDCWNTEG SYDCVCSPGY EPVSGAKTFK NESENTCQDM
121TFSTWTPPPG VHSQTLSRFF DKVQDLGRDY KPGLANNTIQ SILQALDELL EAPGDLETLP
181RLQQHCVASH LLDGLEDVLR GLSKNLSNGL LNFSYPAGTE LSLEVQKQVD RSVTLRQNQA
241VMQLDWNQAQ KSGDPGPSVV GLVSIPGMGK LLAEAPLVLE PEKQMLLHET HQGLLQDGSP
301ILLSDVISAF LSNNDTQNLS SPVTFTFSHR SVIPRQKVLC VFWEHGQNGC GHWATTGCST
361IGTRDTSTIC RCTHLSSFAV LMAHYDVQEE DPVLTVITYM GLSVSLLCLL LAALTFLLCK
421AIQNTSTSLH LQLSLCLFLA HLLFLVAIDQ TGHKVLCSII AGTLHYLYLA TLTWMLLEAL
481YLFLTARNLT VVNYSSINRF MKKLMFPVGY GVPAVTVAIS AASRPHLYGT PSRCWLQPEK
541GFIWGFLGPV CAIFSVNLVL FLVTLWILKN RLSSLNSEVS TLRNTRMLAF KATAQLFILG
601CTWCLGILQV GPAARVMAYL FTIINSLQGV FIFLVYCLLS QQVREQYGKW SKGIRKLKTE
661SEMHTLSSSA KADTSKPSTV N
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

681 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.