LSBio
Monoclonal Mouse anti-Human ADGRE2 / EMR2 Antibody (clone 2A1, IHC, WB)

Flow cytometry of human peripheral blood granulocytes with Mouse anti-Human CD312. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Monoclonal Mouse anti-Human ADGRE2 / EMR2 Antibody (clone 2A1, IHC, WB)

Primary AntibodyLSBio Guarantee
Antibody:Mouse Monoclonal
Applications:Flow Cytometry, Immunohistochemistry (general), Immunohistochemistry, Frozen, Immunoprecipitation, Western Blot
Reactivity:Human
Format:Liquid
See all Human ADGRE2 / EMR2 antibodies →
$455.00each
Catalog Number: LS-C188288-0.2

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Overview

Description
EMR2 antibody LS-C188288 is an unconjugated mouse monoclonal antibody to human EMR2 (ADGRE2). Validated for Flow, IHC, IP and WB.
Application
FC, IHC, IHC-F, IP, WB
Reactivity
Hu
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Monoclonal Antibodies
Alternative Names
CD312 antigen | EMR2 | EGF-like module receptor 2 | CD312
Recommended Dilution: FC
1:50 - 1:200
Recommended Dilution: IHC-F
1:50 - 1:200
UniProt Number
Q9UHX3
NCBI GenBank
NM_013447 | NP_038475.2
SwissProt
Q9UHX3

Target

Target
Human ADGRE2 / EMR2
Antigen Species
Human
Epitope
Recognizes human EMR2, a member of the epidermal growth factor-seven transmembrane (EGF-TM7) family of proteins, which is closely related to CD97. EMR2, also known as CD312, is predominantly expressed on myeloid dendritic cells, monocytes and tissue macrophages. Various isoforms of EMR2 have been documented. The ligand for the largest isoform of EMR2 has recently been identified as chrondroitin sulphate, which binds to the fourth EGF-like module of EMR2. Clone 2A1 specifically recognizes the stalk region of EMR2.
Gene Name
ADGRE2

Antibody

Host Species
Mouse
Clonality
Monoclonal
Clone
2A1
Isotype
IgG1

Immunogen

Immunogen
NIH-3T3 cells stably transfected with EMR2 (EGF1-5) cDNA.
Immunogen Type
Cell-Based Immunization

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS, 0.09% Sodium Azide
Concentration
1 mg/mL
Preservative
Yes
Purification Method
Protein G Affinity

Storage & Handling

Storage Conditions
Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.
Recommended Storage Buffer
PBS

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.