Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ADGRE2 / EMR2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G105943-100
Catalog Number: LS-G105943
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human EMR2(EMR2) (NM_152918), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CD312 antigen | EMR2 | EGF-like module receptor 2 | CD312
- Origin Species
- Human
- NCBI GenBank
- NM_013447
- SwissProt
- Q9UHX3
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 74.8 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MGGRVFLVFL AFCVWLTLPG AETQDSRGCA RWCPQDSSCV NATACRCNPG FSSFSEIITT61PMETCDDINE CATLSKVSCG KFSDCWNTEG SYDCVCSPGY EPVSGAKTFK NESENTCQDM121TFSTWTPPPG VHSQTLSRFF DKVQDLGRDY KPGLANNTIQ SILQALDELL EAPGDLETLP181RLQQHCVASH LLDGLEDVLR GLSKNLSNGL LNFSYPAGTE LSLEVQKQVD RSVTLRQNQA241VMQLDWNQAQ KSGDPGPSVV GLVSIPGMGK LLAEAPLVLE PEKQMLLHET HQGLLQDGSP301ILLSDVISAF LSNNDTQNLS SPVTFTFSHR SVIPRQKVLC VFWEHGQNGC GHWATTGCST361IGTRDTSTIC RCTHLSSFAV LMAHYDVQEE DPVLTVITYM GLSVSLLCLL LAALTFLLCK421AIQNTSTSLH LQLSLCLFLA HLLFLVAIDQ TGHKVLCSII AGTLHYLYLA TLTWMLLEAL481YLFLTARNLT VVNYSSINRF MKKLMFPVGY GVPAVTVAIS AASRPHLYGT PSRCWLQPEK541GFIWGFLGPV CAIFSVNLVL FLVTLWILKN RLSSLNSEVS TLRNTRMLAF KATAQLFILG601CTWCLGILQV GPAARVMAYL FTIINSLQGV FIFLVYCLLS QQVREQYGKW SKGIRKLKTE661SEMHTLSSSA KADTSKPSTV NTRTRPLEQKLISEEDLAANDILDYKDDDDKV
681 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.