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Human ACS5 / ACSL5 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human ACS5 / ACSL5 Protein (Over-Expression Lysate Myc-DDK (Flag))

Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all ACSL5 antibodies →
$220.00each
Catalog Number: LS-G82624-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human ACSL5 (NM_203380) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ACS2 | ACSL5 | ACS5 | FACL5 | Fatty acid coenzyme A ligase 5 | Phosphatidylinositol 4-kinase | LACS 5
Origin Species
Human
NCBI GenBank
NM_016234
SwissProt
Q9ULC5

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
75.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MLFIFNFLFS PLPTPALICI LTFGAAIFLW LITRPQPVLP LLDLNNQSVG IEGGARKGVS
61QKNNDLTSCC FSDAKTMYEV FQRGLAVSDN GPCLGYRKPN QPYRWLSYKQ VSDRAEYLGS
121CLLHKGYKSS PDQFVGIFAQ NRPEWIISEL ACYTYSMVAV PLYDTLGPEA IVHIVNKADI
181AMVICDTPQK ALVLIGNVEK GFTPSLKVII LMDPFDDDLK QRGEKSGIEI LSLYDAENLG
241KEHFRKPVPP SPEDLSVICF TSGTTGDPKG AMITHQNIVS NAAAFLKCVE HAYEPTPDDV
301AISYLPLAHM FERIVQAVVY SCGARVGFFQ GDIRLLADDM KTLKPTLFPA VPRLLNRIYD
361KVQNEAKTPL KKFLLKLAVS SKFKELQKGI IRHDSFWDKL IFAKIQDSLG GRVRVIVTGA
421APMSTSVMTF FRAAMGCQVY EAYGQTECTG GCTFTLPGDW TSGHVGVPLA CNYVKLEDVA
481DMNYFTVNNE GEVCIKGTNV FKGYLKDPEK TQEALDSDGW LHTGDIGRWL PNGTLKIIDR
541KKNIFKLAQG EYIAPEKIEN IYNRSQPVLQ IFVHGESLRS SLVGVVVPDT DVLPSFAAKL
601GVKGSFEELC QNQVVREAIL EDLQKIGKES GLKTFEQVKA IFLHPEPFSI ENGLLTPTLK
661AKRGELSKYF RTQIDSLYEH IQD
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

683 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.