Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
FBX09 / FBXO9 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G114471-100
Catalog Number: LS-G114471
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FBXO9 (NM_012347), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- F-box protein Fbx9 | FBX09 | FBX9 | FBXO9 | NY-REN-57 | VCIA1 | DJ341E18.2 | F-box only protein 9 | F-box protein 9
- Origin Species
- Human
- NCBI GenBank
- NM_012347
- SwissProt
- Q9UK97
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 52.1 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MPDIIWVFPP QAEAEEDCHS DTVRADDDEE NESPAETDLQ AQLQMFRAQW MFELAPGVSS61SNLENRPCRA ARGSLQKTSA DTKGKQEQAK EEKARELFLK AVEEEQNGAL YEAIKFYRRA121MQLVPDIEFK ITYTRSPDGD GVGNSYIEDN DDDSKMADLL SYFQQQLTFQ ESVLKLCQPE181LESSQIHISV LPMEVLMYIF RWVVSSDLDL RSLEQLSLVC RGFYICARDP EIWRLACLKV241WGRSCIKLVP YTSWREMFLE RPRVRFDGVY ISKTTYIRQG EQSLDGFYRA WHQVEYYRYI301RFFPDGHVMM LTTPEEPQSI VPRLRTRNTR TDAILLGHYR LSQDTDNQTK VFAVITKKKE361EKPLDYKYRY FRRVPVQEAD QSFHVGLQLC SSGHQRFNKL IWIHHSCHIT YKSTGETAVS421AFEIDKMYTP LFFARVRSYT AFSERPLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
447 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.