Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
FBX09 / FBXO9 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G108222-100
Catalog Number: LS-G108222
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FBXO9 (NM_033481), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- F-box protein Fbx9 | FBX09 | FBX9 | FBXO9 | NY-REN-57 | VCIA1 | DJ341E18.2 | F-box only protein 9 | F-box protein 9
- Origin Species
- Human
- NCBI GenBank
- NM_012347
- SwissProt
- Q9UK97
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 47.2 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MFRAQWMFEL APGVSSSNLE NRPCRAARGS LQKTSADTKG KQEQAKEEKA RELFLKAVEE61EQNGALYEAI KFYRRAMQLV PDIEFKITYT RSPDGDGVGN SYIEDNDDDS KMADLLSYFQ121QQLTFQESVL KLCQPELESS QIHISVLPME VLMYIFRWVV SSDLDLRSLE QLSLVCRGFY181ICARDPEIWR LACLKVWGRS CIKLVPYTSW REMFLERPRV RFDGVYISKT TYIRQGEQSL241DGFYRAWHQV EYYRYIRFFP DGHVMMLTTP EEPQSIVPRL RTRNTRTDAI LLGHYRLSQD301TDNQTKVFAV ITKKKEEKPL DYKYRYFRRV PVQEADQSFH VGLQLCSSGH QRFNKLIWIH361HSCHITYKST GETAVSAFEI DKMYTPLFFA RVRSYTAFSE RPLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
403 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.