LSBio
CCR3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CCR3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all CCR3 antibodies →
$504.00each
Catalog Number: LS-G128084-100

For bulk orders or custom formulations:

Request a Quote

Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CCR3 (NM_001164680), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
C-C chemokine receptor type 3 | B-chemokine receptor | CD193 | CD193 antigen | CCR3 | Chemokine c-c motif receptor 3 | C-C chemokine receptor 3 | CKR3 | CC-CKR-3 | CCR-3 | CMKBR3 | Eosinophil eotaxin receptor | Eotaxin receptor | C-C CKR-3 | CC chemokine receptor 3
Origin Species
Human
NCBI GenBank
NM_001837
SwissProt
P51677

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
42.9 kda
Protein Type
Lysate
Amino Acid Sequence
1MPFGIRMLLR AHKPGRSEMT TSLDTVETFG TTSYYDDVGL LCEKADTRAL MAQFVPPLYS
61LVFTVGLLGN VVVVMILIKY RRLRIMTNIY LLNLAISDLL FLVTLPFWIH YVRGHNWVFG
121HGMCKLLSGF YHTGLYSEIF FIILLTIDRY LAIVHAVFAL RARTVTFGVI TSIVTWGLAV
181LAALPEFIFY ETEELFEETL CSALYPEDTV YSWRHFHTLR MTIFCLVLPL LVMAICYTGI
241IKTLLRCPSK KKYKAIRLIF VIMAVFFIFW TPYNVAILLS SYQSILFGND CERSKHLDLV
301MLVTEVIAYS HCCMNPVIYA FVGERFRKYL RHFFHRHLLM HLGRYIPFLP SEKLERTSSV
361SPSTAEPELS IVF
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

373 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.