Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CARMA1 / CARD11 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G102046-100
Catalog Number: LS-G102046
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CARD11 (NM_032415), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Card-maguk protein 1 | CARMA1 | Carma 1 | BIMP3 | CARD11
- Origin Species
- Human
- NCBI GenBank
- NM_032415
- SwissProt
- Q9BXL7
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 133.1 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MDDYMETLKD EEDALWENVE CNRHMLSRYI NPAKLTPYLR QCKVIDEQDE DEVLNAPMLP61SKINRAGRLL DILHTKGQRG YVVFLESLEF YYPELYKLVT GKEPTRRFST IVVEEGHEGL121THFLMNEVIK LQQQMKAKDL QRCELLARLR QLEDEKKQMT LTRVELLTFQ ERYYKMKEER181DSYNDELVKV KDDNYNLAMR YAQLSEEKNM AVMRSRDLQL EIDQLKHRLN KMEEECKLER241NQSLKLKNDI ENRPKKEQVL ELERENEMLK TKNQELQSII QAGKRSLPDS DKAILDILEH301DRKEALEDRQ ELVNRIYNLQ EEARQAEELR DKYLEEKEDL ELKCSTLGKD CEMYKHRMNT361VMLQLEEVER ERDQAFHSRD EAQTQYSQCL IEKDKYRKQI RELEEKNDEM RIEMVRREAC421IVNLESKLRR LSKDSNNLDQ SLPRNLPVTI ISQDFGDASP RTNGQEADDS STSEESPEDS481KYFLPYHPPQ RRMNLKGIQL QRAKSPISLK RTSDFQAKGH EEEGTDASPS SCGSLPITNS541FTKMQPPRSR SSIMSITAEP PGNDSIVRRY KEDAPHRSTV EEDNDSGGFD ALDLDDDSHE601RYSFGPSSIH SSSSSHQSEG LDAYDLEQVN LMFRKFSLER PFRPSVTSVG HVRGPGPSVQ661HTTLNGDSLT SQLTLLGGNA RGSFVHSVKP GSLAEKAGLR EGHQLLLLEG CIRGERQSVP721LDTCTKEEAH WTIQRCSGPV TLHYKVNHEG YRKLVKDMED GLITSGDSFY IRLNLNISSQ781LDACTMSLKC DDVVHVRDTM YQDRHEWLCA RVDPFTDHDL DMGTIPSYSR AQQLLLVKLQ841RLMHRGSREE VDGTHHTLRA LRNTLQPEEA LSTSDPRVSP RLSRASFLFG QLLQFVSRSE901NKYKRMNSNE RVRIISGSPL GSLARSSLDA TKLLTEKQEE LDPESELGKN LSLIPYSLVR961AFYCERRRPV LFTPTVLAKT LVQRLLNSGG AMEFTICKSD IVTRDEFLRR QKTETIIYSR1021EKNPNAFECI APANIEAVAA KNKHCLLEAG IGCTRDLIKS NIYPIVLFIR VCEKNIKRFR1081KLLPRPETEE EFLRVCRLKE KELEALPCLY ATVEPDMWGS VEELLRVVKD KIGEEQRKTI1141WVDEDQLSGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
1147 aa + 34 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.