Immunocytochemistry analysis of CARMA1 using anti-CARMA1, pAb (AL220) . . Method: Human T cells (clone 6396p5.1.2) were incubated with anti-CD3, mAb for 20 min. on ice, followed by incubation with secondary goat anti-mouse for 15 min. at 4°C or 37°C. Cells were stained for CD3 (green), phosphotyrosine (red) and CARMA1 (1:200 dilution; blue) and analyzed by confocal microscopy.
CARMA1 / CARD11 Antibody (aa1-221)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Immunocytochemistry, Immunohistochemistry (general), Western Blot |
| Reactivity: | Human, Mouse |
| Format: | Liquid |
$458.00each
Catalog Number: LS-C344843-100
Catalog Number: LS-C344843
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- Description
- CARD11 antibody LS-C344843 is an unconjugated rabbit polyclonal antibody to CARD11 (CARMA1) (aa1-221) from human. It is reactive with human and mouse. Validated for ICC, IHC and WB.
- Application
- ICC, IHC, WB
- Reactivity
- Hu, Ms
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- Card-maguk protein 1 | CARMA1 | Carma 1 | BIMP3 | CARD11
- Recommended Dilution: IF/ICC
- 1:200
- Recommended Dilution: WB
- 1:1000
- UniProt Number
- Q9BXL7
- NCBI GenBank
- NM_032415 | NP_115791.3
- SwissProt
- Q9BXL7
Target
- Target
- Human CARMA1 / CARD11
- Antigen Species
- Human
- Epitope
- Recognizes human and mouse CARMA1. Detects a band of ~130Dka by Western blot.
- Gene Name
- CARD11
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
Immunogen
- Immunogen
- N-Terminus human CARMA1 (aa 1-221 containing the CARD domain).
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, 0.02% Sodium Azide, 10% Glycerol
- Preservative
- Yes
- Purification Method
- Protein A Affinity
Storage & Handling
- Storage Conditions
- Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS