Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CALD1 / Caldesmon Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G108410-100
Catalog Number: LS-G108410
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Caldesmon(CALD1) (NM_033157), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CALD1 | Caldesmon | Caldesmon 1 | HCAD | LCAD | H-CAD | NAG22 | CDM | L-CAD
- Origin Species
- Human
- NCBI GenBank
- NM_004342
- SwissProt
- Q05682
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 65.4 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MDDFERRREL RRQKREEMRL EAERIAYQRN DDDEEEAARE RRRRARQERL RQKQEEESLG61QVTDQVEVNA QNSVPDEEAK TTTTNTQVEG DDEAAFLERL ARREERRQKR LQEALERQKE121FDPTITDASL SLPSRRMQND TAENETTEKE EKSESRQERY EIEETETVTK SYQKNDWRDA181EENKKEDKEK EEEEEEKPKR GSIGENQGEE KGTKVQAKRE KLQEDKPTFK KEEIKDEKIK241KDKEPKEEVK SFMDRKKGFT EVKSQNGEFM THKLKHTENT FSRPGGRASV DTKEAEGAPQ301VEAGKRLEEL RRRRGETESE EFEKLKQKQQ EAALELEELK KKREERRKVL EEEEQRRKQE361EADRKLREEE EKRRLKEEIE RRRAEAAEKR QKMPEDGLSD DKKPFKCFTP KGSSLKIEER421AEFLNKSVQK SSGVKSTHQA AIVSKIDSRL EQYTSAIEGT KSAKPTKPAA SDLPVPAEGV481RNIKSMWEKG NVFSSPTAAG TPNKETAGLK VGVSSRINEW LTKTPDGNKS PAPKPSDLRP541GDVSSKRNLW EKQSVDKVTS PTKVTRTRPLEQKLISEEDLAANDILDYKDDDDKV
564 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.