Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CALD1 / Caldesmon Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G108401-100
Catalog Number: LS-G108401
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Caldesmon(CALD1) (NM_033139), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CALD1 | Caldesmon | Caldesmon 1 | HCAD | LCAD | H-CAD | NAG22 | CDM | L-CAD
- Origin Species
- Human
- NCBI GenBank
- NM_004342
- SwissProt
- Q05682
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 64.1 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MLGGSGSHGR RSLAALSQIA YQRNDDDEEE AARERRRRAR QERLRQKQEE ESLGQVTDQV61EVNAQNSVPD EEAKTTTTNT QVEGDDEAAF LERLARREER RQKRLQEALE RQKEFDPTIT121DASLSLPSRR MQNDTAENET TEKEEKSESR QERYEIEETE TVTKSYQKND WRDAEENKKE181DKEKEEEEEE KPKRGSIGEN QGEEKGTKVQ AKREKLQEDK PTFKKEEIKD EKIKKDKEPK241EEVKSFMDRK KGFTEVKSQN GEFMTHKLKH TENTFSRPGG RASVDTKEAE GAPQVEAGKR301LEELRRRRGE TESEEFEKLK QKQQEAALEL EELKKKREER RKVLEEEEQR RKQEEADRKL361REEEEKRRLK EEIERRRAEA AEKRQKMPED GLSDDKKPFK CFTPKGSSLK IEERAEFLNK421SVQKSSGVKS THQAAIVSKI DSRLEQYTSA IEGTKSAKPT KPAASDLPVP AEGVRNIKSM481WEKGNVFSSP TAAGTPNKET AGLKVGVSSR INEWLTKTPD GNKSPAPKPS DLRPGDVSSK541RNLWEKQSVD KVTSPTKVTRTRPLEQKLISEEDLAANDILDYKDDDDKV
558 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.