LSBio
AKT3 Protein (Over-Expression Lysate Myc-DDK (Flag))

AKT3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all AKT3 antibodies →
$725.00each
Catalog Number: LS-G100558-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human AKT3 (NM_005465), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
AKT3 | AKT3/PKBgamma | PRKBG | PKB gamma | Protein kinase Akt-3 | RAC-gamma | MPPH | PKB-GAMMA | PKBG | Protein kinase B gamma | RAC-PK-gamma | STK-2
Origin Species
Hu
NCBI GenBank
NM_005465
SwissProt
Q9Y243

Protein

Expression System
HEK293
Fusion Tag
FLAG, Myc
Molecular Weight
55.6 kda
Protein Type
Lysate
Amino Acid Sequence
1MSDVTIVKEG WVQKRGEYIK NWRPRYFLLK TDGSFIGYKE KPQDVDLPYP LNNFSVAKCQ
61LMKTERPKPN TFIIRCLQWT TVIERTFHVD TPEEREEWTE AIQAVADRLQ RQEEERMNCS
121PTSQIDNIGE EEMDASTTHH KRKTMNDFDY LKLLGKGTFG KVILVREKAS GKYYAMKILK
181KEVIIAKDEV AHTLTESRVL KNTRHPFLTS LKYSFQTKDR LCFVMEYVNG GELFFHLSRE
241RVFSEDRTRF YGAEIVSALD YLHSGKIVYR DLKLENLMLD KDGHIKITDF GLCKEGITDA
301ATMKTFCGTP EYLAPEVLED NDYGRAVDWW GLGVVMYEMM CGRLPFYNQD HEKLFELILM
361EDIKFPRTLS SDAKSLLSGL LIKDPNKRLG GGPDDAKEIM RHSFFSGVNW QDVYDKKLVP
421PFKPQVTSET DTRYFDEEFT AQTITITPPE KYDEDGMDCM DNERRPHFPQ FSYSASGRE
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

479 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconj
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.