Western blot of Mouse anti-AKT3 antibody. Lane 1: GST Tagged recombinant AKT1. Lane 2: GST Tagged recombinant AKT2. Lane 3: GST Tagged recombinant AKT3. Load: 25 ng per lane. Primary antibody: AKT3 antibody at 1:1000 for overnight at 4C. Secondary antibody: Peroxidase mouse secondary antibody at 1:40000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 78 kDa for AKT3. Other band(s): none.

AKT3 Antibody (Internal, clone 25F6.F6.D8)

Primary AntibodyLSBio Guarantee

Antibody:	Mouse Monoclonal
Applications:	Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Immunohistochemistry (general), Western Blot
Reactivity:	Human
Format:	Liquid

$586.00each

Catalog Number: LS-C153788-100

Unit Size

Quantity

For bulk orders or custom formulations:

Overview

Description: AKT3 antibody LS-C153788 is an unconjugated mouse monoclonal antibody to human AKT3 (Internal). Validated for ELISA, Flow, IHC and WB.
Application: ELISA, FC, IHC, WB
Reactivity: Hu
Intended Use: Research Use Only
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Monoclonal Antibodies
Alternative Names: AKT3 | AKT3/PKBgamma | PRKBG | PKB gamma | Protein kinase Akt-3 | RAC-gamma | MPPH | PKB-GAMMA | PKBG | Protein kinase B gamma | RAC-PK-gamma | STK-2
Recommended Dilution: ELISA: 1:2000 - 1:10000
Recommended Dilution: WB: 1:500 - 1:2000
UniProt Number: Q9Y243
NCBI GenBank: NM_005465 | NP_005456.1
SwissProt: Q9Y243

Target

Target: Human AKT3
Antigen Species: Human
Epitope: The antibody detects both unphosphorylated and phosphorylated forms of the protein. Anti-AKT3 antibody was purified from ascites by Protein A chromatography. Cross reactivity with AKT3 from other species has not been determined, however, the sequence of the immunogen shows 100% identity to human, mouse, and rat, therefore, cross reactivity is expected. Cross-reactivity with AKT2 and AKT has not been determined.
Gene Name: AKT3

Antibody

Host Species: Mouse
Clonality: Monoclonal
Clone: 25F6.F6.D8
Isotype: IgG1
Light Chain: Kappa Light Chain

Immunogen

Immunogen: Anti-AKT3 Antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to internal residues of human AKT3 protein.
Immunogen Type: Synthetic Peptide (Linear)

Format

Conjugate/Label: Unconjugated
Format: Liquid
Formulation: 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration: 1 mg/mL
Preservative: Yes
Purification Method: Protein A Affinity

Storage & Handling

Storage Conditions: Store at -20°C. Aliquot to avoid freeze-thaw cycles. Store undiluted.
Recommended Storage Buffer: 0.02 M Potassium Phosphate|0.15 M NaCl