Western Blot of Mouse monoclonal anti-AKT3 antibody. Lane 1: GST-AKT1. Lane 2: GST-AKT2. Lane 3: GST-AKT3. Load: 50 ng per lane. Primary antibody: anti-AKT3 antibody at 1:1000 for overnight at 4 degrees C. Secondary antibody: Rabbit secondary antibody anti mouse at 1:40,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4 degrees C. Predicted/Observed size: 85 kDa for GST-AKT3. This image was taken for the unmodified form of this product. Other forms have not been tested.

AKT3 Antibody (Internal, clone 9A5.H9.G7)

Primary AntibodyLSBio Guarantee

Antibody:	Mouse Monoclonal
Applications:	Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Immunofluorescence, Immunohistochemistry (general), Western Blot
Reactivity:	Human
Format:	Liquid

$2652.00each

Catalog Number: LS-C209202-1

Unit Size

Quantity

For bulk orders or custom formulations:

Overview

Description: AKT3 antibody LS-C209202 is an unconjugated mouse monoclonal antibody to human AKT3 (Internal). Validated for ELISA, Flow, IF, IHC and WB.
Application: ELISA, FC, IF, IHC, WB
Reactivity: Hu
Intended Use: Research Use Only
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Monoclonal Antibodies
Alternative Names: AKT3 | AKT3/PKBgamma | PRKBG | PKB gamma | Protein kinase Akt-3 | RAC-gamma | MPPH | PKB-GAMMA | PKBG | Protein kinase B gamma | RAC-PK-gamma | STK-2
Recommended Dilution: ELISA: 1:2000 - 1:10000
Recommended Dilution: WB: 1:500 - 1:2000
UniProt Number: Q9Y243
NCBI GenBank: NM_005465 | NP_005456.1
SwissProt: Q9Y243

Target

Target: Human AKT3
Antigen Species: Human
Epitope: The antibody detects both unphosphorylated and phosphorylated forms of the protein. Anti-AKT3 antibody was purified from ascites by Protein A chromatography. Cross reactivity with AKT3 from other species has not been determined, however, the sequence of the immunogen shows 100% identity to human, mouse, and rat, therefore, cross reactivity is expected. Cross-reactivity with AKT2 and AKT has not been determined.
Gene Name: AKT3

Antibody

Host Species: Mouse
Clonality: Monoclonal
Clone: 9A5.H9.G7
Isotype: IgG1
Light Chain: Kappa Light Chain

Immunogen

Immunogen: Anti-AKT3 Antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to internal residues of human AKT3 protein.
Immunogen Type: Synthetic Peptide (Linear)

Format

Conjugate/Label: Unconjugated
Format: Liquid
Formulation: 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl
Concentration: 0.7 mg/mL
Preservative: No
Purification Method: Protein A Affinity

Storage & Handling

Storage Conditions: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Centrifuge product before removing cap. Only dilute immediately prior to use.
Recommended Storage Buffer: 0.02 M Potassium Phosphate|0.15 M NaCl