Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ADAM2 / Fertilin Beta Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G118562-100
Catalog Number: LS-G118562
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ADAM2 (NM_001464), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ADAM2 | ADAM 2 | CRYN1 | CRYN2 | CT15 | ADAM metallopeptidase domain 2 | FTNB | Fertilin | Fertilin beta | Fertilin subunit beta | PH-30 | PH-30b | PH30 | PH30-beta | Cancer/testis antigen 15
- Origin Species
- Human
- NCBI GenBank
- NM_001464
- SwissProt
- Q99965
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 82.3 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MWRVLFLLSG LGGLRMDSNF DSLPVQITVP EKIRSIIKEG IESQASYKIV IEGKPYTVNL61MQKNFLPHNF RVYSYSGTGI MKPLDQDFQN FCHYQGYIEG YPKSVVMVST CTGLRGVLQF121ENVSYGIEPL ESSVGFEHVI YQVKHKKADV SLYNEKDIES RDLSFKLQSV EPQQDFAKYI181EMHVIVEKQL YNHMGSDTTV VAQKVFQLIG LTNAIFVSFN ITIILSSLEL WIDENKIATT241GEANELLHTF LRWKTSYLVL RPHDVAFLLV YREKSNYVGA TFQGKMCDAN YAGGVVLHPR301TISLESLAVI LAQLLSLSMG ITYDDINKCQ CSGAVCIMNP EAIHFSGVKI FSNCSFEDFA361HFISKQKSQC LHNQPRLDPF FKQQAVCGNA KLEAGEECDC GTEQDCALIG ETCCDIATCR421FKAGSNCAEG PCCENCLFMS KERMCRPSFE ECDLPEYCNG SSASCPENHY VQTGHPCGLN481QWICIDGVCM SGDKQCTDTF GKEVEFGPSE CYSHLNSKTD VSGNCGISDS GYTQCEADNL541QCGKLICKYV GKFLLQIPRA TIIYANISGH LCIAVEFASD HADSQKMWIK DGTSCGSNKV601CRNQRCVSSS YLGYDCTTDK CNDRGVCNNK KHCHCSASYL PPDCSVQSDL WPGGSIDSGN661FPPVAIPARL PERRYIENIY HSKPMRWPFF LFIPFFIIFC VLIAIMVKVN FQRKKWRTED721YSSDEQPESE SEPKGTRTRPLEQKLISEEDLAANDILDYKDDDDKV
735 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.