Fumarase Assay Kit (Colorimetric)
Assay KitLSBio Guarantee
| Assay Format | Enzyme Activity Assay |
| Format: | Kit |
$679.00each
Catalog Number: LS-K269-100
Catalog Number: LS-K269
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- Description
- FUMARASE (or fumarate hydratase) (EC 4.2.1.2) is an enzyme that catalyzes the reversible hydration/dehydration reaction of fumarate to malate. Fumarase exists in two isoforms: a cytosolic and mitochondrial form. In the citric acid cycle, it facilitates a transition step in the production of energy in the form of NADH. Fumarase deficiency in humans results in early brain development problems and is characterized by poor feeding, hypotonia, failure to thrive, etc. This non-radioactive, colorimetric fumarase assay is based on the reduction of the tetrazolium salt MTT in a NADH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at 565 nm. The increase in absorbance at 565 nm is proportional to the enzyme activity.
- Target
- Fumarase
- Category
- Assay Kits > All Assay Kits
- Alternative Names
- FH | Fumarase | Fumarate hydratase | MCUL1 | HLRCC | LRCC | MCL
Assay
- Detection Method
- Colorimetric
- Assay Type
- Quantitative
- Assay Format
- Enzyme Activity Assay
- Compatible Sample Types
- Cell Culture Supernatant, Cells, Plasma, Serum, Tissue Homogenate
- Detection Wavelength (Colorimetric)
- 565 nm
Format
- Format
- Kit
- Kit Components
- Assay Buffer, Enzyme A, NAD\/MTT, Enzyme B, Substrate, Calibrator
Storage & Handling
- Storage Conditions
- Store at -20°C.
Documentation
- Documents
- Document
