Aconitase Assay Kit (Colorimetric)
Assay KitLSBio Guarantee
| Assay Format | Enzyme Activity Assay |
| Format: | Kit |
$490.00each
Catalog Number: LS-K199-100
Catalog Number: LS-K199
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- ACONITASE (ACONITATE HYDRATASE) is an enzyme in the citric acid (TCA) cycle that catalyzes the conversion of citrate to isocitrate. The activity of aconitase depends largely upon the iron-sulfur [Fe4S4] 2+ cluster. Related diseases include aconitase deficiency (e.g. myopathy and exercise intolerance), Friedreich's ataxia and diabetes. This aconitase assay measures the isocitrate generated as a product of the aconitase reaction. The isocitrate is then oxidized producing NADPH and the oxidation product. The NADPH converts the dye to an intense violet color with an absorption maximum at 565 nm. The increase in absorbance at 565 nm is directly proportional to aconitase activity.
- Target
- Aconitase
- Category
- Assay Kits > All Assay Kits
- Alternative Names
- ACONS | Aconitase | Aconitase 1, soluble | Aconitate hydratase | Citrate hydro-lyase | ACO1 | IREB1 | IRE-BP 1 | IREBP | IRP1 | Ferritin repressor protein | IREBP1 | Iron regulatory protein 1
Assay
- Detection Method
- Colorimetric
- Assay Type
- Quantitative
- Assay Format
- Enzyme Activity Assay
- Compatible Sample Types
- Other Biological Sample
- Detection Wavelength (Colorimetric)
- 565 nm
Format
- Format
- Kit
- Kit Components
- Assay Buffer, Enzyme A, NADP\/MTT, Enzyme B, standard Substrate
Storage & Handling
- Storage Conditions
- Store at -20°C.
Documentation
- Documents
- Document
