Isocitrate Assay Kit (Fluorometric)
Assay KitLSBio Guarantee
| Assay Format | Biochemical Assay |
| Format: | Kit |
$521.00each
Catalog Number: LS-K219-100
Catalog Number: LS-K219
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- Description
- ISOCITRATE (ISOCITRIC ACID) is a substrate in the citric acid (TCA) cycle. Isocitrate is formed by the isomerization of citrate catalyzed by the enzyme aconitase. Isocitrate is oxidized by isocitrate dehydrogenase producing Alpha-ketoglutarate and generating NADPH. Isocitrate is commonly found in many fruits and vegetables and their processed products. Industrially, isocitrate is used as a marker to identify the quality and purity of fruit juices. This isocitrate assay measures the NADPH generated from the oxidation of isocitrate. The NADPH reduces a probe into a highly fluorescent product. The fluorescence intensity of this product, measured at lambda ex/em = 530/585 nm, is proportional to the isocitrate concentration in the sample.
- Target
- Isocitrate
- Category
- Assay Kits > All Assay Kits
Assay
- Detection Method
- Fluorescent / Fluorometric
- Assay Type
- Quantitative
- Assay Format
- Biochemical Assay
- Compatible Sample Types
- Food Sample, Milk, Other Biological Sample, Plasma, Saliva, Serum, Tears, Tissue Homogenate, Urine
Format
- Format
- Kit
- Kit Components
- Assay Buffer, Enzyme A, NADP, Enzyme B, standard Probe
Storage & Handling
- Storage Conditions
- Store at -20°C.
Documentation
- Documents
- Document
