LSBio
IHC-plus™ TNF Alpha Antibody (clone 52B83)

Anti-TNF Alpha antibody IHC of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

IHC-plus™ TNF Alpha Antibody (clone 52B83)

Primary AntibodyLSBio Guarantee
Antibody:Mouse Monoclonal
Applications:Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Immunohistochemistry (general), Immunohistochemistry, Frozen, Immunohistochemistry, Paraffin, Western Blot
Reactivity:Guinea Pig, Human, Mouse, Non-Human Primate
Format:Liquid
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$495.00each
Catalog Number: LS-B2123-50

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Overview

Description
TNF Alpha antibody LS-B2123 is an unconjugated mouse monoclonal antibody to TNF Alpha from human. It is reactive with human, mouse, guinea pig and other species. Validated for ELISA, Flow, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Application
ELISA, FC, IHC, IHC-F, IHC-P, WB
Reactivity
GP, Hu, Ms, NHP
Intended Use
Research Use Only
Guarantees
This product carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Monoclonal Antibodies
Alternative Names
APC1 protein | Cachectin | DIF | TNF | TNF Alpha | Tumor necrosis factor-alpha | TNF-alpha | TNF-a | TNFA | Tumor necrosis factor | TNF, macrophage-derived | TNF, monocyte-derived | TNFSF2
Recommended Dilution: FC
1:50
Recommended Dilution: IHC-F
1:50
Recommended Dilution: IHC-P
2.5 µg/ml
Recommended Dilution: WB
1:50
UniProt Number
P01375
NCBI GenBank
NM_000594 | NP_000585.2
SwissProt
P01375

Target

Target
TNF Alpha
Antigen Species
Human
Specificity
Recognizes human, guinea pig, monkey, mouse native and recombinant TNF-alpha, including soluble (17kD) and membrane (26kD) forms. Does not cross-react with TNF-beta, lymphotoxin and receptor bound TNF-alpha
Epitope
Recognizes human native and recombinant TNF-alpha. Reacts with soluble (17kD) and membrane (26kD) human TNF-alpha. Does not cross-react with TNF-beta, lymphotoxin and receptor bound TNF-alpha.
Gene Name
TNF

Antibody

Host Species
Mouse
Clonality
Monoclonal
Clone
52B83
Origin Species
Human

Immunogen

Immunogen
Human TNFa.

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS, 0.02% Sodium Azide, 0.1% BSA
Concentration
0.1 mg/mL
Animal Free
No
Carrier-Free
No
Preservative
Yes
Purification Method
Other

Storage & Handling

Storage Conditions
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
PBS

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.