LSBio
TNF Alpha Antibody

Western Blot of Mouse TNFa Antibody. Lane 1: Mouse TNFa. Load: 50 ng per lane. Primary antibody: Mouse TNFa antibody at 1:1,000 overnight at 4°C. Secondary antibody: HRP rabbit secondary antibody at 1:40,000 for 30 min at RT.

TNF Alpha Antibody

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunoprecipitation, Neutralization, Radioimmunoassay, Western Blot
Reactivity:Mouse
Format:Liquid
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$1250.00each
Catalog Number: LS-C18838-1

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Overview

Description
TNF Alpha antibody LS-C18838 is an unconjugated rabbit polyclonal antibody to mouse TNF Alpha. Validated for ELISA, IHC, IP, Neut, RIA and WB. Cited in 1 publication.
Application
ELISA, IHC, IP, Neut, RIA, WB
Reactivity
Ms
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
APC1 protein | Cachectin | DIF | TNF | TNF Alpha | Tumor necrosis factor-alpha | TNF-alpha | TNF-a | TNFA | Tumor necrosis factor | TNF, macrophage-derived | TNF, monocyte-derived | TNFSF2
Recommended Dilution: ELISA
1:1000 - 1:5000
Recommended Dilution: WB
1:500 - 1:2000
UniProt Number
P01375
NCBI GenBank
NM_000594 | NP_000585.2
SwissProt
P01375

Target

Target
Mouse TNF Alpha
Antigen Species
Mouse
Epitope
The antibody is directed against mature 17,000 MW mouse TNFa and is useful in determining its presence in various assays. The antibody does not recognize mouse TNFβ (lymphotoxin).
Gene Name
TNF

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Isotype
IgG

Immunogen

Immunogen
This antibody was produced from whole rabbit serum prepared by repeated immunizations with recombinant mouse TNFa produced in E.coli.
Immunogen Type
Recombinant Immunogen

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration
1 mg/mL
Preservative
Yes
Purification Method
Unpurified (Serum / Ascites / Supernatant)

Storage & Handling

Storage Conditions
Store vial at -20°C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
Recommended Storage Buffer
0.02 M Potassium Phosphate|0.15 M NaCl
Adiponectin inhibits the activation of lung fibroblasts and pulmonary fibrosis by regulating the nuclear factor kappa B (NF-?B) pathway. Xin Wang, Jian Yang, Liangquan Wu, Chunran Tong, Ying Zhu, Wei Cai, Bing Wan, Xiuwei Zhang. Bioengineered. 2022 April;13:10098-10110. PubMed: 35435119 PMC: PMC9162013
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