Intracellular flow cytometry of TLR4 in human PBMCs using TLR4 antibody at 0.5 ug/10^6 cells. The shaded histogram represents cells without TLR4 antibody; green represents the isotype control; red represents TLR4 antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.

TLR4 Antibody (clone HTA125, PE)

Primary AntibodyLSBio Guarantee

Antibody:	Mouse Monoclonal
Applications:	Flow Cytometry
Reactivity:	Dog, Human
Format:	Liquid

$425.00each

Catalog Number: LS-C148165-0.1

Unit Size

Quantity

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Overview

Description: TLR4 antibody LS-C148165 is a PE-conjugated mouse monoclonal antibody to TLR4 from human. It is reactive with human and dog. Validated for Flow.
Application: FC
Reactivity: Can, Hu
Intended Use: Research Use Only
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Monoclonal Antibodies
Alternative Names: CD284 | CD284 antigen | Homolog of Drosophila toll | TLR-4 | TOLL | ARMD10 | TLR4 | Toll-like receptor 4 | HToll
UniProt Number: O00206
NCBI GenBank: NM_138554
SwissProt: O00206

Target

Target: Human TLR4
Antigen Species: Human
Epitope: This recognizes the human Toll like receptor 4 (TLR4) cell surface antigen. TLR4, also known as CD284, has been demonstrated to act as a receptor for LPS on human monocytes and macrophages. TLR4 signalling of LPS stimulation requires the presence of the MD-2 molecule. TLR4 is weakly expressed by resting cells, but is upregulated following stimulation with LPS. This has been demonstrated to block activation of monocytes with LPS.
Gene Name: TLR4

Antibody

Host Species: Mouse
Clonality: Monoclonal
Clone: HTA125
Isotype: IgG2a
Light Chain: Kappa Light Chain

Immunogen

Immunogen: This antibody was developed by immunizing mice with Ba/F3 cell line expressing human TLR4 cell surface antigen (9).
Immunogen Type: Cell-Based Immunization

Format

Conjugate/Label: Phycoerythrin
Format: Liquid
Formulation: PBS, 0.05% Sodium Azide, 0.05% BSA
Volume: 0.1 Milliliter
Preservative: Yes
Purification Method: Protein G Affinity

Storage & Handling

Storage Conditions: May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for up to 6 months at 4°C. Protect from light.
Recommended Storage Buffer: PBS