LSBio
PathPlus™ TLR4 Antibody (aa100-200, clone 76B357.1)

Anti-TLR4 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

PathPlus™ TLR4 Antibody (aa100-200, clone 76B357.1)

Primary AntibodyLSBio Guarantee
Antibody:Mouse Monoclonal
Applications:Flow Cytometry, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity:Human, Mouse, Rat
Format:Liquid
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$383.00each
Catalog Number: LS-B2070-50

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Overview

Description
TLR4 antibody LS-B2070 is an unconjugated mouse monoclonal antibody to TLR4 (aa100-200) from human. It is reactive with human, mouse and rat. Validated for Flow, IHC and WB. Cited in 2 publications.
Application
FC, IHC, IHC-P, WB
Reactivity
Hu, Ms, Rt
Intended Use
Research Use Only
Guarantees
This product carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Monoclonal Antibodies
Alternative Names
CD284 | CD284 antigen | Homolog of Drosophila toll | TLR-4 | TOLL | ARMD10 | TLR4 | Toll-like receptor 4 | HToll
Recommended Dilution: IHC-P
5 µg/ml
Recommended Dilution: WB
1 - 3 µg/ml
UniProt Number
O00206
NCBI GenBank
NM_138554
SwissProt
O00206

Target

Target
TLR4
Antigen Species
Human
Specificity
A portion of amino acids 100-200 of human TLR4.
Epitope
The amino acid sequence used has 100% identity in human, chimp, baboon, Chinese hamster, and rat; 93% identity in bovine and pig; 85% identity in sheep and 78% identity in cat, horse and mouse.
Gene Name
TLR4

Antibody

Host Species
Mouse
Clonality
Monoclonal
Clone
76B357.1
Light Chain
Kappa Light Chain
Origin Species
Human

Immunogen

Immunogen
Synthetic peptide from human TLR4.
Immunogen Type
Synthetic Peptide (Linear)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS, 0.05% Sodium Azide
Concentration
1 mg/mL
Animal Free
No
Carrier-Free
Yes
Preservative
Yes
Purification Method
Protein G Affinity

Storage & Handling

Storage Conditions
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
PBS

Tissue expression of Toll-like receptors 2 and 4 in sporadic human colorectal cancer. Nihon-Yanagi Y, Terai K, Murano T, Matsumoto T, Okazumi S. Cancer immunology, immunotherapy : CII. 2013 Jan;61:71-7.

PubMed: 21845432 · PMC: PMC3249192


RAPID COMMUNICATION: TLR4 expressed but with reduced functionality on equine B lymphocytes. Alayna N Hay, Ashley Potter, Leah Kasmark, Jing Zhu, Caroline M Leeth. Journal of animal science. 2019 Apr;97:2175-2180.

PubMed: 30901382 · PMC: PMC6488325

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