Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~60.4 kDa SMAD4 protein using Rabbit Anti-SMAD4 Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: Cervical Cancer cell line (HeLa) lysate. Load: 10 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-SMAD4 Polyclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~60.4 kDa.

SMAD4 Antibody (APC)

Primary AntibodyLSBio Guarantee

Antibody:	Rabbit Polyclonal
Applications:	Immunohistochemistry (general), Western Blot
Reactivity:	Human
Format:	Liquid

$488.00each

Catalog Number: LS-C774951-100

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Overview

Description: SMAD4 antibody LS-C774951 is an APC-conjugated rabbit polyclonal antibody to human SMAD4. Validated for IHC and WB.
Application: IHC, WB
Reactivity: Hu
Intended Use: Research Use Only
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names: DPC4 | HSMAD4 | MYHRS | SMAD 4 | MAD homolog 4 | SMAD family member 4 | SMAD4 | JIP | MADH4 | Mothers against DPP homolog 4
Recommended Dilution: IHC: 1:50
Recommended Dilution: WB: 1:1000
UniProt Number: Q13485
NCBI GenBank: NM_005359 | NP_005350.1
SwissProt: Q13485

Target

Target: Human SMAD4
Antigen Species: Human
Epitope: Detects 60 kDa.
Gene Name: SMAD4

Antibody

Host Species: Rabbit
Clonality: Polyclonal
Origin Species: Human

Immunogen

Immunogen: Synthetic peptide of Human SMAD4 (200-300 aa), conjugated to Keyhole Limpet Haemocyanin (KLH).
Immunogen Type: Synthetic Peptide (Linear)