LSBio
Polyclonal Rabbit anti-Human SMAD4 Antibody (WB)

Anti-SMAD4 - Western Blot. Western blot using affinity purified anti-SMAD4 to detect over-expressed SMAD4 in transfected COS cells (lane 2). Lane 1 contains lysate from mock transfected COS cells. A doublet band is seen in the SMAD4 transfected lysate with the upper band (arrowhead) representing SMAD4 and the lower band being non-specific staining. The membrane was probed with the primary antibody at a 1:1000 dilution. Personal Communication Kathleen Flanders, CCR-NCI, Bethesda, MD.

Polyclonal Rabbit anti-Human SMAD4 Antibody (WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Western Blot
Reactivity:Human
Format:Liquid
See all Human SMAD4 antibodies →
$579.00each
Catalog Number: LS-C60121-100

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Overview

Description
SMAD4 antibody LS-C60121 is an unconjugated rabbit polyclonal antibody to SMAD4 from human. It is reactive with human, mouse and xenopus. Validated for ELISA and WB.
Application
ELISA, WB
Reactivity
Hu
Predicted Reactivity
Mouse, Xenopus
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
DPC4 | HSMAD4 | MYHRS | SMAD 4 | MAD homolog 4 | SMAD family member 4 | SMAD4 | JIP | MADH4 | Mothers against DPP homolog 4
Recommended Dilution: ELISA
1:15000 - 1:60000
Recommended Dilution: WB
1:500 - 1:2000
UniProt Number
Q13485
NCBI GenBank
NM_005359 | NP_005350.1
SwissProt
Q13485

Target

Target
Human SMAD4
Antigen Species
Human
Epitope
A BLAST analysis was used to suggest cross-reactivity with SMAD4 protein from human, mouse and Xenopus based on 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.
Gene Name
SMAD4

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Isotype
IgG

Immunogen

Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the carboxy terminus of human SMAD4 protein.
Immunogen Type
Synthetic Peptide (Linear)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration
1.2 mg/mL
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Recommended Storage Buffer
0.02 M Potassium Phosphate|0.15 M NaCl

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