LSBio
Rabbit Anti-Mouse IgG2a Secondary Antibody

Western Blot of Rabbit anti-Mouse IgG2a antibody. Lane 1: Mouse IgG1. Lane 2: Mouse IgG2a. Lane 3: Mouse IgG2b. Lane 4: Mouse IgG3. Lane 5: Mouse IgM. Load: 5 ng per lane. Primary antibody: Mouse IgG2a antibody at 1 µg/mL for overnight at 4°C. Secondary antibody: Rabbit secondary antibody at 1:40,000 for 30 min at RT.

Rabbit Anti-Mouse IgG2a Secondary Antibody

Secondary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunoprecipitation, Other, Western Blot
Reactivity:Mouse
Format:Liquid
See all Mouse IgG2a antibodies →
$360.00each
Catalog Number: LS-C60755-1

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Overview

Description
Mouse IgG2a antibody LS-C60755 is an unconjugated rabbit polyclonal antibody. Validated for ELISA, IHC and WB.
Application
ELISA, IHC, IP, Other, WB
Reactivity
Ms
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Secondary Antibodies
Recommended Dilution: ELISA
1:15000 - 1:30000
Recommended Dilution: IHC
1:2000 - 1:10000
Recommended Dilution: WB
1:4000 - 1:20000

Target

Target
Mouse IgG2a
Antigen Species
Mouse
Epitope
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Mouse Serum and Mouse IgG2a. Specificity was confirmed by ELISA at less than 1% cross-reactivity against other mouse heavy or light chain isotypes.

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Isotype
IgG

Immunogen

Immunogen
Mouse IgG2a heavy chain.

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration
1.1 mg/mL
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Store at 4°C.
Recommended Storage Buffer
0.02 M Potassium Phosphate|0.15 M NaCl

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.