Polyclonal Goat anti-Goat Biotin Antibody (FITC, IF)
Catalog Number: LS-C758060-1
1 mg / $322.00
Antibody: Goat Polyclonal
Applications: Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Immunofluorescence
Reactivity: Goat
Format: Liquid
Western Blot of ATTO 647N conjugated Goat anti-Mouse IgG2a Pre-adsorbed secondary antibody. Lane 1: Mouse IgG2a. Lane 2: none. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: ATTO 647N goat secondary antibody at 1:1,000 for 60 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 55 kDa, 55 kDa for Mouse IgG2a. Other band(s): none. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Polyclonal Goat Anti-Mouse IgG2a Secondary Antibody (Atto 647N, IF, WB)
Secondary AntibodyLSBio Guarantee
| Antibody: | Goat Polyclonal |
| Applications: | Flow Cytometry, Immunofluorescence, Other, Western Blot |
| Reactivity: | Mouse |
| Format: | Lyophilized |
$482.00each
Catalog Number: LS-C209485-500
Catalog Number: LS-C209485
For bulk orders or custom formulations:
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- Description
- Mouse IgG2a antibody LS-C209485 is an Atto 647N-conjugated goat polyclonal antibody. Validated for FLISA, Flow, IF and WB.
- Application
- FC, IF, Other, WB
- Reactivity
- Ms
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Secondary Antibodies
- Recommended Dilution: FC
- 1:500 - 1:2500
- Recommended Dilution: IF/ICC
- 1:5000
- Recommended Dilution: WB
- 1:4000 - 1:20000
Target
- Target
- Mouse IgG2a
- Antigen Species
- Mouse
- Epitope
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse Serum and Mouse IgG2a. Specificity was confirmed by ELISA at less than 1% cross-reactivity against other mouse heavy or light chain isotypes. No reaction was observed against Bovine, Human, and Rabbit Serum Proteins. Specificity was confirmed by ELISA at less than 1% of target signal.
Antibody
- Host Species
- Goat
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- Mouse IgG2a heavy chain.
Format
- Conjugate/Label
- Atto 647N
- Format
- Lyophilized
- Formulation
- Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Centrifuge product before removing cap. Only dilute immediately prior to use.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl
