LSBio
Goat Anti-Mouse IgG2a Secondary Antibody (TRITC)

Dot Blot of Goat anti-Mouse IgG2a Antibody Rhodamine Conjugated Pre-absorbed. Antigen: Mouse IgG2a. Load: Lane 1 - 200 ng Lane 2 - 66.7 ng Lane 3 - 22.2 ng Lane 4 - 7.41 ng Lane 5 - 2.47 ng. Primary antibody: n/a. Secondary antibody: Goat anti-Mouse IgG2a Antibody Rhodamine Conjugated Pre-absorbed at 1:1,000 overnight at 4°C.

Goat Anti-Mouse IgG2a Secondary Antibody (TRITC)

Secondary AntibodyLSBio Guarantee
Antibody:Goat Polyclonal
Applications:Dot Blot, Immunofluorescence, Immunohistochemistry (general), Other, Western Blot
Reactivity:Mouse
Format:Lyophilized
See all Mouse IgG2a antibodies →
$376.00each
Catalog Number: LS-C209433-1

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Overview

Description
Mouse IgG2a antibody LS-C209433 is a TRITC-conjugated goat polyclonal antibody. Validated for DB, FLISA, IF, IHC and WB.
Application
DB, IF, IHC, Other, WB
Reactivity
Ms
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Secondary Antibodies
Recommended Dilution: IF/ICC
1:1000 - 1:5000
Recommended Dilution: IHC
1:1000 - 1:5000
Recommended Dilution: WB
1:2000 - 1:10000

Target

Target
Mouse IgG2a
Antigen Species
Mouse
Epitope
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse Serum and Mouse IgG2a. Specificity was confirmed by ELISA at less than 1% cross-reactivity against other mouse heavy or light chain isotypes. No reaction was observed against Bovine, Human, and Rabbit Serum Proteins. Specificity was confirmed by ELISA at less than 1% of target signal.

Antibody

Host Species
Goat
Clonality
Polyclonal
Isotype
IgG

Immunogen

Immunogen
Mouse IgG2a heavy chain.

Format

Conjugate/Label
Tetramethylrhodamine Isothiocyanate
Format
Lyophilized
Formulation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration
1 mg/mL
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Centrifuge product before removing cap. Only dilute immediately prior to use.
Recommended Storage Buffer
0.02 M Potassium Phosphate|0.15 M NaCl

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.